Anti-eIF4EBP1 (phospho T36) 抗体 (ab47365)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T36)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-eIF4EBP1 (phospho T36) antibody
eIF4EBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF4EBP1 (phospho T36) -
由来種
Rabbit -
特異性
ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human eIF4EBP1 aa 1-100 (phospho T36).
Database link: Q13541 -
ポジティブ･コントロール
- MDA-MB-435 cell extracts; Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
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| WB |
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
配列類似性
Belongs to the eIF4E-binding protein family. -
翻訳後修飾
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Entrez Gene: 116636 Rat
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- SwissProt: Q62622 Rat
- Unigene: 411641 Human
see all -
別名
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
画像
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Western blot - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)All lanes : Anti-eIF4EBP1 (phospho T36) antibody (ab47365)
Lane 1 : MDA-MB-435 cells,
treated with EGF (200 ng/ml, 30min)
Lane 2 : MDA-MB-435 cells, untreated
Predicted band size: 13 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab47365 は 10 報の論文で使用されています。
- Li Y et al. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng 2022:2373580 (2022). PubMed: 35035814
- Zhang X et al. hnRNP C modulates MERS-CoV and SARS-CoV-2 replication by governing the expression of a subset of circRNAs and cognitive mRNAs. Emerg Microbes Infect 11:519-531 (2022). PubMed: 35060842
- Zhao J et al. Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion. Exp Mol Med 53:1207-1217 (2021). PubMed: 34385569
- Wang L et al. Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway. Cell Cycle 19:376-389 (2020). PubMed: 31924125
- Liu J et al. Valsartan ameliorates high glucose-induced peritoneal ?brosis by blocking mTORC1 signaling. Exp Biol Med (Maywood) 245:983-993 (2020). PubMed: 32408765
- Lai WS & Ding YL GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway. Int J Mol Med 43:1939-1950 (2019). PubMed: 30864685
- Liu J et al. Mammalian Target of Rapamycin Complex 1 Activation Disrupts the Low-Density Lipoprotein Receptor Pathway: A Novel Mechanism for Extracellular Matrix Accumulation in Human Peritoneal Mesothelial Cells. Am J Nephrol 48:357-368 (2018). PubMed: 30423569
- Lu J et al. MicroRNA-218-5p as a Potential Target for the Treatment of Human Osteoarthritis. Mol Ther 25:2676-2688 (2017). PubMed: 28919376
- Jiang Z et al. Blocking mammalian target of rapamycin alleviates bone cancer pain and morphine tolerance via µ-opioid receptor. Int J Cancer 138:2013-20 (2016). WB ; Rat . PubMed: 26566757
- Wang S et al. Predictive value of phosphorylated mammalian target of rapamycin for disease-free survival in breast cancer patients receiving neoadjuvant chemotherapy. Oncol Lett 8:2642-2648 (2014). PubMed: 25364442