AB2606

Anti-eIF4EBP1 抗体

Name: Anti-eIF4EBP1 抗体 (ab2606) | Abcam
Brand: Abcam Limited
SKU: AB2606
Price: 81000 JPY
Availability: InStock
Rating: 5 (4 reviews)

Anti-eIF4EBP1 antibody

(4 Reviews)

(39 Publications)

Rabbit Polyclonal eIF4EBP1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1.

別名を表示する

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (AB2606)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (AB2606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection : DAB.

Unknown

Western blot - Anti-eIF4EBP1 antibody (AB2606)

ab2606 at a 1 : 4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
after exposure to high (lane 1) and low (lane 2) concentrations of leucine. ab2606 at a 1 : 4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.

All lanes:

Western blot - Anti-eIF4EBP1 antibody (ab2606)

Predicted band size: 13 kDa

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Western blot - Anti-eIF4EBP1 antibody (AB2606)

All lanes:

Western blot - Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eIF4EBP1 protein (<a href='/products/proteins-peptides/recombinant-human-eif4ebp1-protein-ab60007'>ab60007</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human eIF4EBP1 protein (<a href='/products/proteins-peptides/recombinant-human-eif4ebp1-protein-ab60007'>ab60007</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

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Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human EIF4EBP1. The exact immunogen used to generate this antibody is proprietary information.

Q13541

特異性

This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/20000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

Affinity purified using the immunising peptideimmobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.

Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

See full target information EIF4EBP1

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文献 (39)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2250 PubMed40050638

2025

Cryptic mitochondrial DNA mutations coincide with mid-late life and are pathophysiologically informative in single cells across tissues and species.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair P Green,Florian Klimm,Aidan S Marshall,Rein Leetmaa,Juvid Aryaman,Aurora Gómez-Durán,Patrick F Chinnery,Nick S Jones

The Journal of biological chemistry 300:107542 PubMed38992436

2024

Activation of nemo-like kinase in diamond blackfan anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Wilkes,Aya Shibuya,Y Lucy Liu,Kailen Mark,Jaqueline Mercado,Mallika Saxena,Ryan S Sathianathen,Hye Na Kim,Bertil Glader,Paraic Kenny,Kathleen M Sakamoto

PLoS biology 21:e3002355 PubMed37943958

2023

Nonsense mutation suppression is enhanced by targeting different stages of the protein synthesis process.

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Species

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Amnon Wittenstein,Michal Caspi,Ido Rippin,Orna Elroy-Stein,Hagit Eldar-Finkelman,Sven Thoms,Rina Rosin-Arbesfeld

Frontiers in nutrition 10:1098715 PubMed36969813

2023

Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway.

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Unspecified application

Species

Unspecified reactive species

Yingao Qi,Tenghui Zheng,Xinghong Liu,Siwang Yang,Qihui Li,Jiayuan Shao,Xiangfang Zeng,Wutai Guan,Shihai Zhang

Journal of animal science and technology 64:922-936 PubMed36287778

2022

Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line.

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Unspecified application

Species

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Xusheng Dong,Chen Liu,Jialin Miao,Xueyan Lin,Yun Wang,Zhonghua Wang,Qiuling Hou

Autophagy 19:1087-1099 PubMed35998113

2022

NPPA/atrial natriuretic peptide is an extracellular modulator of autophagy in the heart.

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Unspecified application

Species

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Maurizio Forte,Simona Marchitti,Flavio Di Nonno,Rosita Stanzione,Leonardo Schirone,Maria Cotugno,Franca Bianchi,Sonia Schiavon,Salvatore Raffa,Danilo Ranieri,Salvatore Fioriniello,Floriana Della Ragione,Maria Rosaria Torrisi,Roberto Carnevale,Valentina Valenti,Francesco Versaci,Giacomo Frati,Carmine Vecchione,Massimo Volpe,Speranza Rubattu,Sebastiano Sciarretta

Clinical, cosmetic and investigational dermatology 15:643-651 PubMed35444442

2022

Enhanced Activation of mTOR Signaling Pathway Was Found in the Hypertrophic and Nodular Lesions of Port Wine Stains.

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Meng-Nan Xu,Qian Wang,Min Wang,Yuan Xu,Si-Ming Yuan

Oncology letters 23:70 PubMed35069879

2022

CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect.

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Species

Unspecified reactive species

Wenyu Yang,Yanchun Wang,Chunhui Tao,Yunhai Li,Shan Cao,Xiqian Yang

Frontiers in veterinary science 8:756375 PubMed34869729

2021

Intracellular Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells.

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Unspecified application

Species

Unspecified reactive species

Yuhao Chen,Yuze Ma,Qiang Ji,Xiaoru Yang,Xue Feng,Ruiyuan Yao,Xiaoou Cheng,Tingting Li,Yanfeng Wang,Zhigang Wang

Experimental & molecular medicine 53:1207-1217 PubMed34385569

2021

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion.

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Unspecified application

Species

Unspecified reactive species

Jingyi Zhao,Bingyan Li,Yongxia Ren,Tiansong Liang,Juan Wang,Suna Zhai,Xiqian Zhang,Pengcheng Zhou,Xiangxian Zhang,Yuanyuan Pan,Fangfang Gao,Sulan Zhang,Liming Li,Yongqiang Yang,Xiaoyu Deng,Xiaole Li,Linhui Chen,Daoke Yang,Yingjuan Zheng

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