Name: Anti-eIF4E antibody [Y449]
Brand: RabMAb
SKU: ab33768
Rating: 5 (8 reviews)

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Western blot - Anti-eIF4E antibody [Y449] (ab33768)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y449] to eIF4E
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-eIF4E antibody [Y449]
eIF4E 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [Y449] to eIF4E
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human eIF4E aa 150-250. The exact sequence is proprietary.
ポジティブ･コントロール
- WB: HEK-293, HepG2, NIH/3T3, RAW 264.7, and C6 cell lysates; IHC-P: Human ovarian cancer tissue. Mouse and rat testis tissues; ICC/IF: RAW 264.7 cells; Flow Cyt (intra): HEK293 cells; IP: NIH/3T3 cell lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

Y449
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-eIF4E antibody [Y449] - BSA and Azide free (ab246346)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HEK-293 whole cell lysate (ab7902)
Recombinant Protein
- Recombinant Human eIF4E protein (ab56276)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab33768の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		Use at an assay dependent concentration.
WB	(7)	1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). For unpurified use at 1/5000 - 1/20000.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP		1/30.

特記事項
Flow Cyt (Intra) 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). For unpurified use at 1/5000 - 1/20000.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP 1/30.

機能

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
配列類似性

Belongs to the eukaryotic initiation factor 4E family.
翻訳後修飾

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Target information above from: UniProt accession P06730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Entrez Gene: 117045 Rat
- Omim: 133440 Human
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- SwissProt: P63074 Rat
- Unigene: 249718 Human
- Unigene: 3941 Mouse
- Unigene: 11275 Rat
see all
別名
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
- eIF 4F 25 kDa subunit antibody
- EIF 4F antibody
- eIF-4E antibody
- eIF-4F 25 kDa subunit antibody
- eIF4E antibody
- EIF4E1 antibody
- EIF4EL1 antibody
- EIF4F antibody
- Eukaryotic translation initiation factor 4 E antibody
- Eukaryotic translation initiation factor 4E antibody
- Eukaryotic translation initiation factor 4E like 1 antibody
- IF4E_HUMAN antibody
- Messanger RNA Cap Binding Protein eIF 4E antibody
- MGC111573 antibody
- mRNA cap binding protein antibody
- mRNA cap-binding protein antibody
see all

Western blot - Anti-eIF4E antibody [Y449] (ab33768)

All lanes : Anti-eIF4E antibody [Y449] (ab33768) at 1/1000 dilution

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time:
Lane 1: 20 seconds.
Lanes 2-5: 8 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody [Y449] (ab33768)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling eIF4E with ab33768 at a dilution of 1/500. Antigen retrieval was performed ab93684, Tris/EDTA buffer, pH 9. A ready to use goat anti-rabbit IgG H&L (HRP Polymer) was used as the secondary antibody. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody [Y449] (ab33768)

Immunocytochemistry/ Immunofluorescence analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling eIF4E with Purified ab33768 at 1/50 (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-eIF4E antibody [Y449] (ab33768)

Intracellular Flow Cytometry analysis of HEK-293 (Human embryonickidney epithelial cell) cells labeling eIF4E with Purified ab33768 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-eIF4E antibody [Y449] (ab33768)

eIF4E was immunoprecipitated from 10 µg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with ab33768 at a 1/30 dilution. Western blot was performed from the immunoprecipitate using ab33768 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10 µg (Input).
Lane 2: ab33768 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab33768 in NIH/3T3 whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody [Y449] (ab33768)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling eIF4E with ab33768 at a dilution of 1/500. Antigen retrieval was performed ab93684, Tris/EDTA buffer, pH 9. A ready to use goat anti-rabbit IgG H&L (HRP Polymer) was used as the secondary antibody. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody [Y449] (ab33768)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling eIF4E with ab33768 at a dilution of 1/500. Antigen retrieval was performed ab93684, Tris/EDTA buffer, pH 9. A ready to use goat anti-rabbit IgG H&L (HRP Polymer) was used as the secondary antibody. Counter stained with Hematoxylin.
Anti-eIF4E antibody [Y449] (ab33768)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab33768 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab33768 は 4 報の論文で使用されています。

Rao A et al. The translation initiation factor homolog eif4e1c regulates cardiomyocyte metabolism and proliferation during heart regeneration. Development 150:N/A (2023). PubMed: 37306388
Yang X et al. RETRACTED: The role of ATG-7 contributes to pulmonary hypertension by impacting vascular remodeling. J Mol Cell Cardiol 157:1-13 (2021). PubMed: 33819456
Jhun M et al. CD103 Deficiency Promotes Autism (ASD) and Attention-Deficit Hyperactivity Disorder (ADHD) Behavioral Spectra and Reduces Age-Related Cognitive Decline. Front Neurol 11:557269 (2020). PubMed: 33424735
Oblinger JL et al. Overexpression of eIF4F components in meningiomas and suppression of meningioma cell growth by inhibiting translation initiation. Exp Neurol 299:299-307 (2018). PubMed: 28610844

レビューと Q&A

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レビューを送る質問を送る

1-8 of 8 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Zebrafish Tissue sections (Heart)

Permeabilization

Specification

Heart

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Aaron Goldman

Verified customer

投稿 Jun 13 2023

Application

Western blot

Sample

Zebrafish Cell lysate - whole cell (Heart)

Gel Running Conditions

Reduced Denaturing (4-20% Bis-Tris)

Loading amount

20 µg

Specification

Heart

Blocking step

ECL Advance as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Aaron Goldman

Verified customer

投稿 Jun 08 2023

Application

Western blot

Sample

Dog Cell lysate - whole cell (MDCK cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

MDCK cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2022

Application

Western blot

Sample

Monkey Cell lysate - whole cell (COS-1 cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

COS-1 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2022

Application

Western blot

Sample

Rat Cell lysate - whole cell (3611-RF cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

3611-RF cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (MEFs)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

MEFs

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

HeLa cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

mouse embryonic stem cells (J1)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 10 2018

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (4)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]

Western blot abreview for Anti-eIF4E antibody [Y449]