Anti-eIF4B 抗体 (ab186856)
Key features and details
- Rabbit polyclonal to eIF4B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-eIF4B antibody
eIF4B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF4B -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human eIF4B aa 352-611 (C terminal).
Database link: P23588 -
ポジティブ･コントロール
- MCF7, 22RV-1, HeLa, 293T and M21 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186856の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 69 kDa.
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 69 kDa. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. -
配列類似性
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. - Information by UniProt
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参照データベース
- Entrez Gene: 1975 Human
- Omim: 603928 Human
- SwissProt: P23588 Human
- Unigene: 648394 Human
- Unigene: 702041 Human
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別名
- 2310046H11Rik antibody
- AL024095 antibody
- C85189 antibody
see all
画像
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Western blot - Anti-eIF4B antibody (ab186856)All lanes : Anti-eIF4B antibody (ab186856) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : 22RV-1 cell extract
Lane 3 : HeLa cell extract
Lane 4 : 293T cell extract
Lane 5 : M21 cell extract
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody (ab186856)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling eIF4B with ab186856 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody (ab186856)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186856. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab186856 は論文での使用が確認できていません。