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リコンビナントRabMAb

Anti-eIF4A1 抗体 [EPR14506] (ab185946)

Reviews (1) Submit a question References (2)
Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
  • Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
  • Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)
  • Anti-eIF4A1 antibody [EPR14506] (ab185946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14506] to eIF4A1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-eIF4A1 antibody [EPR14506]
    eIF4A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14506] to eIF4A1

  • 由来種

    Rabbit

  • 特異性

    The immunogen used for this product shares 93% homology with eIF4A2. Cross-reactivity with this protein has not been confirmed experimentally.

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, 293, HeLa, MCF7, mouse Raw264.7, mouse NIH 3T3, rat C6 and rat Pc12 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185946の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP
1/30 - 1/40.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP
1/30 - 1/40.

ターゲット情報

  • 機能

    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

  • 配列類似性

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ATP-dependent RNA helicase eIF4A-1 antibody
    • DDX2 antibody
    • DDX2A antibody
    • eIF 4A I antibody
    • eIF-4A-I antibody
    • EIF4A antibody
    • eIF4A I antibody
    • eIF4A-I antibody
    • EIF4A1 antibody
    • Eukaryotic initiation factor 4A-I antibody
    • Eukaryotic initiation factor 4AI antibody
    • eukaryotic translation initiation factor 4A, isoform 1 antibody
    • Eukaryotic translation initiation factor 4A1 antibody
    • IF4A1_HUMAN antibody
    see all

画像

  • Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/1000 dilution

    Lane 1 : rat C6 cell lysate
    Lane 2 : mouse Raw264.7 cell lysate
    Lane 3 : rat PC12 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling eIF4A1 using ab185946 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Anti-eIF4A1 antibody [EPR14506] (ab185946)

参考文献 (2)

ab185946 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185946 は 2 報の論文で使用されています。

  • Li F  et al. Astragaloside IV exhibits anti-tumor function in gastric cancer via targeting circRNA dihydrolipoamide S-succinyltransferase (circDLST)/miR-489-3p/ eukaryotic translation initiation factor 4A1(EIF4A1) pathway. Bioengineered 13:10111-10122 (2022). PubMed: 35435117
  • Panhale A  et al. CAPRI enables comparison of evolutionarily conserved RNA interacting regions. Nat Commun 10:2682 (2019). PubMed: 31213602

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-eIF4A1 antibody [EPR14506]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Mar 05 2018

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