Anti-eIF3l 抗体 (ab154527)
Key features and details
- Rabbit polyclonal to eIF3l
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-eIF3l antibody
eIF3l 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF3l -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow, Human, Xenopus laevis, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to Human eIF3l aa 330-564.
Database link: Q9Y262 -
ポジティブ･コントロール
- Mouse brown adipose and white adipose whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
配列類似性
Belongs to the eIF-3 subunit L family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 514449 Cow
- Entrez Gene: 51386 Human
- Entrez Gene: 223691 Mouse
- Entrez Gene: 380037 Xenopus laevis
- Entrez Gene: 394577 Xenopus tropicalis
- SwissProt: Q3ZCK1 Cow
- SwissProt: Q9Y262 Human
- SwissProt: Q8QZY1 Mouse
see all -
別名
- eIEF associated protein HSPC021 antibody
- EIF3EIP antibody
- eif3l antibody
see all
画像
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Western blot - Anti-eIF3l antibody (ab154527)All lanes : Anti-eIF3l antibody (ab154527) at 1/1000 dilution
Lane 1 : Mouse brown adipose whole cell lysate
Lane 2 : Mouse white adipose whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
7.5% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154527 は論文での使用が確認できていません。