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AB264229

Anti-eIF3B 抗体

Anti-eIF3B antibody

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Rabbit Polyclonal EIF3B antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human EIF3B aa 1-50.

別名を表示する

EIF3S9, EIF3B, Eukaryotic translation initiation factor 3 subunit B, eIF3b, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog, eIF-3-eta, eIF3 p110, eIF3 p116, hPrt1

1 Images
Western blot - Anti-eIF3B antibody (AB264229)
  • WB

Supplier Data

Western blot - Anti-eIF3B antibody (AB264229)

Detected with chemilimuniscence with exposure time of 30 seconds.

4-8% SDS-PAGE

All lanes:

Western blot - Anti-eIF3B antibody (ab264229) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 92 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human EIF3B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P55884

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代替製品

Primary Antibodies

AB225173

PE Anti-eIF3B antibody [EPR5804]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-eIF3B antibody [EPR5804] (AB225173)

  • 0
  • 0
Primary Antibodies

AB133601

Anti-eIF3B antibody [EPR5804]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (AB133601)

  • 0
  • 1
Primary Antibodies

AB124778

Anti-eIF3B antibody [EPR5805]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805] (AB124778)

  • 0
  • 0
Primary Antibodies

AB248000

Anti-eIF3B antibody [EPR5805] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805] - BSA and Azide free (AB248000)

  • 0
  • 0
Primary Antibodies

AB238921

Anti-eIF3B antibody [EPR5804] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB315966

Anti-EIF3F antibody [EPR24363-61]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-EIF3F antibody [EPR24363-61] (AB315966)

  • 0
  • 0
Primary Antibodies

AB128996

Anti-eIF3A antibody [EPR5819(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody [EPR5819(2)] (AB128996)

  • 0
  • 0
Primary Antibodies

AB151550

Anti-RPS10 antibody [EPR8545]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS10 antibody [EPR8545] (AB151550)

  • 0
  • 0
Primary Antibodies

AB251743

Anti-EIF3H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 3 subunit B (eIF3B) also known as EIF3S9 or p116 plays a central role in the initiation of translation. This protein has a mass of approximately 97 kDa and serves as an important component of the eIF3 complex important for assembling the 40S ribosomal subunit with mRNA and initiator tRNA. eIF3B is ubiquitously expressed in various tissues indicating its fundamental role in cellular protein synthesis.
Biological function summary

EIF3B functions as part of the large eIF3 complex which consists of 13 subunits. This complex is important for the recruitment of mRNA to the 40S ribosomal subunit playing an essential role in the start of translation. eIF3B directly contributes to stabilizing the interaction between mRNA and the ribosome promoting the accurate and efficient formation of the translation initiation complex. This function highlights the importance of eIF3B in regulating cellular protein levels.

Pathways

EIF3B participates in key processes of mRNA translation and is integral to the translation initiation pathway. It interacts with other translation initiation factors including eIF4F and eIF5 orchestrating the assembly and positioning of the ribosome for translation initiation. Moreover eIF3B is also involved in the mTOR signaling pathway linking nutrient sensing to protein synthesis regulation.

EIF3B has been associated with various types of cancer such as breast and prostate cancer where its increased expression correlates with tumor progression and poor prognosis. The dysfunction of eIF3B affects protein synthesis and cell proliferation contributing to oncogenesis. Furthermore eIF3B's role is connected to the dysregulation of mTOR pathways which is significant in cancer development. Its interaction with other proteins like eIF4E and eIF5 signifies its impact on cancer progression pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815, PubMed : 9388245). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632, PubMed : 9388245). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).
See full target information EIF3B
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