Anti-eIF3A 抗体 (ab86146)
Key features and details
- Rabbit polyclonal to eIF3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-eIF3A antibody
eIF3A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eIF3A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
A region between residue 860 and 910 of human eIF3A using the numbering given in entry NP_003741.1.
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86146の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 167 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 167 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). -
配列類似性
Belongs to the eIF-3 subunit A family.
Contains 1 PCI domain. -
翻訳後修飾
Phosphorylated. Phosphorylation is enhanced upon serum stimulation. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 466216 Chimpanzee
- Entrez Gene: 8661 Human
- Entrez Gene: 13669 Mouse
- Entrez Gene: 292148 Rat
- Omim: 602039 Human
- SwissProt: Q14152 Human
- SwissProt: P23116 Mouse
- SwissProt: G1SMZ5 Rabbit
see all -
別名
- centrosomin homolog antibody
- cytoplasmic protein p167 antibody
- eIF 3 theta antibody
see all
画像
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Western blot - Anti-eIF3A antibody (ab86146)This image is courtesy of an anonymous AbreviewAnti-eIF3A antibody (ab86146) at 1/1000 dilution + Mouse pancreatic cancer whole cell lysate at 20 µg
Secondary
IRDye® 680LT-conjugated donkey anti-mouse IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 167 kDa
Observed band size: 166 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody (ab86146)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling eIF3A with ab86146 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-eIF3A antibody (ab86146)This image is courtesy of an anonymous AbreviewAnti-eIF3A antibody (ab86146) at 1/1000 dilution + Human U2OS osteosarcoma whole cell lysate at 20 µg
Secondary
IRDye® 800CW conjugated donkey anti-rabbit polyclonal at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 167 kDa
Observed band size: 166 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Western blot - Anti-eIF3A antibody (ab86146)All lanes : Anti-eIF3A antibody (ab86146) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 167 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-eIF3A antibody (ab86146)Detection of Human eIF3A by Immunoprecipitation, using ab86146 at 3µg/mg lysate. Image shows immunoprecipitated eIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86146 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab86146 は 8 報の論文で使用されています。
- Chen C et al. Translational and post-translational control of human naïve versus primed pluripotency. iScience 25:103645 (2022). PubMed: 35005567
- Faucher-Giguère L et al. High-grade ovarian cancer associated H/ACA snoRNAs promote cancer cell proliferation and survival. NAR Cancer 4:zcab050 (2022). PubMed: 35047824
- Ye Y et al. Genome-wide identification and characterization of circular RNA m6A modification in pancreatic cancer. Genome Med 13:183 (2021). PubMed: 34798904
- Jin D et al. m6A demethylase ALKBH5 inhibits tumor growth and metastasis by reducing YTHDFs-mediated YAP expression and inhibiting miR-107/LATS2-mediated YAP activity in NSCLC. Mol Cancer 19:40 (2020). PubMed: 32106857
- Liu S et al. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis. Cell 176:491-504.e21 (2019). PubMed: 30612740
- D'Amico D et al. The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging. Mol Cell 73:775-787.e10 (2019). PubMed: 30642763
- Sorci M et al. METTL3 regulates WTAP protein homeostasis. Cell Death Dis 9:796 (2018). WB ; Human . PubMed: 30038300
- Conrad KD et al. MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation. PLoS One 8:e56272 (2013). IP ; Human . PubMed: 23405269