Anti-EIF2S2/EIF2B 抗体 [EPR15833(B)] (ab184549)
![Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)](https://www.abcam.co.jp/ps/products/184/ab184549/Images/ab184549-217167-anti-eif2s2-antibody-epr15833b-western-blot.jpg "Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EIF2S2/EIF2B antibody [EPR15833(B)]
EIF2S2/EIF2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HepG2, HeLa, 293T and Jurkat cell lysates. HeLa and 293 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15833(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/160.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/500.
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| IP |
1/40 - 1/60.
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| WB |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/500. |
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IP
1/40 - 1/60. |
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WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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機能
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
配列類似性
Belongs to the eIF-2-beta/eIF-5 family. - Information by UniProt
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参照データベース
- Entrez Gene: 8894 Human
- Omim: 603908 Human
- SwissProt: P20042 Human
- Unigene: 429180 Human
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別名
- DKFZp686L18198 antibody
- eIF 2 beta antibody
- eIF-2-beta antibody
see all
画像
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Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)All lanes : Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)](https://www.abcam.co.jp/ps/products/184/ab184549/Images/ab184549-217168-anti-eif2s2-antibody-epr15833b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2/EIF2B using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution, counterstained with Dapi.
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![Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)](https://www.abcam.co.jp/ps/products/184/ab184549/Images/ab184549-217175-anti-eif2s2-antibody-epr15833b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)Western blot analysis of EIF2S2/EIF2B in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.
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![Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)](https://www.abcam.co.jp/ps/products/184/ab184549/Images/ab184549-217169-anti-eif2s2-antibody-epr15833b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)Intracellular flow cytometric analysis of2% paraformaldehyde fixed 293 cells labeling EIF2S2/EIF2B using ab184549 at a 1/160 dilution (red) or aRabbit monoclonal IgG control (green).
Secondary antibody: Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
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![Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)](https://www.abcam.co.jp/ps/products/184/ab184549/Images/ab184549-5-benefits-of-recombinant-antibodies.png)
Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab184549 は論文での使用が確認できていません。