Name: Anti-EIF2S1 (phospho S51) antibody [E90]
Brand: RabMAb
SKU: ab32157
Rating: 5 (26 reviews)

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Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
Suitable for: WB, IHC-P, Dot blot
Reacts with: Mouse, Rat, Human, Neurospora crassa

Carrier Free

製品名

Anti-EIF2S1 (phospho S51) antibody [E90]
EIF2S1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, Dot blotmore details
適用なし: Flow Cyt,ICC/IF or IP
種交差性

交差種: Mouse, Rat, Human, Neurospora crassa
交差が予測される動物種: Cow, Pig, Drosophila melanogaster, Monkey, Plants, African green monkey
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- IHC-P: Human liver carcinoma, breast carcinoma, cervical carcinoma, colon adenocarcinoma and hepatocellular carcinoma tissues. WB: HeLa cells treated with Clyculin A and phosphatase whole cell lysate; PC-12 cell lysate. Dot Blot: Antigen peptide.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

E90
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (ab214434)
Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab32157の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(20)	1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P	(2)	1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot		1/500.

特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot 1/500.

追加情報

Is unsuitable for Flow Cyt,ICC/IF or IP.

機能

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
配列類似性

Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
翻訳後修飾

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
細胞内局在

Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
Target information above from: UniProt accession P05198 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 327694 Cow
- Entrez Gene: 32617 Drosophila melanogaster
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P41374 Drosophila melanogaster
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- SwissProt: P68101 Rat
- Unigene: 3157 Drosophila melanogaster
- Unigene: 151777 Human
- Unigene: 196220 Mouse
- Unigene: 1488 Rat
see all
別名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
- EIF 2alpha antibody
- eIF-2-alpha antibody
- eIF-2A antibody
- EIF-2alpha antibody
- EIF2 alpha antibody
- EIF2 antibody
- EIF2A antibody
- EIF2S1 antibody
- Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
- Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
- Eukaryotic translation initiation factor 2 subunit 1 antibody
- Eukaryotic translation initiation factor 2 subunit alpha antibody
- IF2A_HUMAN antibody
see all

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole cell lysates
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with alkaline phosphatase.
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with lambda phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemical analysis of paraffin-embedded human lung cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human lung cancer without alkaline phosphatase treatment (image A). No staining on human lung cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Dot blot analysis of EIF2S1 (pS51) phospho peptide (Lane 1), EIF2S1 non-phospho peptide (Lane 2) with ab32157 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution

Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa treated with Calyculin A whole cell lysates
Lane 3 : HeLa treated with Calyculin A and phosphatase whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking buffer and concentration: 2% BSA/TBST.

Diluting buffer and concentration: 2% BSA /TBST.

Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (image A). No staining on mouse pancreatic cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate

Predicted band size: 36 kDa
Observed band size: 36 kDa

Secondary antibody - anti-rabbit HRP (ab6721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab32157 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32157 は 274 報の論文で使用されています。

Amin PH et al. An RNA stem-loop functions in conjunction with an upstream open reading frame to direct preferential translation in the integrated stress response. J Biol Chem 299:102864 (2023). PubMed: 36596357
Fan Q et al. Brain injury triggers cell-type-specific and time-dependent endoplasmic reticulum stress responses. Glia 71:667-681 (2023). PubMed: 36412235
Shao Y et al. The inhibition of ORMDL3 prevents Alzheimer's disease through ferroptosis by PERK/ATF4/HSPA5 pathway. IET Nanobiotechnol 17:182-196 (2023). PubMed: 36680386
Drino A et al. Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res 51:1326-1352 (2023). PubMed: 36718960
Zimmermann T et al. Cold Atmospheric Plasma Triggers Apoptosis via the Unfolded Protein Response in Melanoma Cells. Cancers (Basel) 15:N/A (2023). PubMed: 36831408

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-10 of 28 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (primary fibroblasts)

Gel Running Conditions

Reduced Denaturing (precast 5-15%)

Loading amount

30 µg

Treatment

300mMol Thaps 1 hr and 5 hrs

Specification

primary fibroblasts

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 15 2024

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (mice Pancreas)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Permeabilization

Specification

mice Pancreas

Blocking step

Bloxall as blocking agent for 20 minute(s) · Concentration: 100%

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 06 2022

Application

Western blot

Sample

Mouse Tissue lysate - whole (Heart, Liver)

Gel Running Conditions

Reduced Denaturing (12% TGX)

Loading amount

20 µg

Specification

Heart, Liver

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 22 2021

Application

Western blot

Sample

Xenopus laevis Cell lysate - whole cell (Unfertilised Xenopus oocytes, stage V-VI)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

0.5 cells

Specification

Unfertilised Xenopus oocytes, stage V-VI

Blocking step

BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Rowena Martin

Verified customer

投稿 Nov 17 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Human Primary Fibroblasts)

Permeabilization

Yes - 0.1% Triton 1h

Specification

Human Primary Fibroblasts

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 21 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (Human Primary Fibroblasts)

Gel Running Conditions

Reduced Denaturing (12% TGX)

Loading amount

10 µg

Treatment

different treatments

Specification

Human Primary Fibroblasts

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 21 2020

Application

Western blot

Sample

Xenopus laevis Tissue lysate - whole (tedpole's head)

Gel Running Conditions

Non-reduced Denaturing (12%)

Loading amount

8 µg

Treatment

10uM octylphenol for 96hrs

Specification

tedpole's head

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Kyung noh Yoon

Verified customer

投稿 May 09 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing (8 %)

Loading amount

20 µg

Treatment

Thapsigargin (0.5 uM)

Specification

HeLa

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Jin Ik Kim

Verified customer

投稿 Apr 20 2019

Application

Western blot

Sample

Pichia pastoris Cell lysate - whole cell (Pichia Pastoris GS115 (his-))

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

4.4 µg

Specification

Pichia Pastoris GS115 (his-)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 07 2018

Application

Western blot

Sample

Rabbit Cell lysate - whole cell (RK13 cells)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

200000 cells

Specification

RK13 cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 25 2017

1-10 of 28 Abreviews or Q&A

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (274)

レビューと Q&A

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Immunocytochemistry/ Immunofluorescence abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]