Anti-EIF2B5 (phospho S539) 抗体 (ab4775)
Key features and details
- Rabbit polyclonal to EIF2B5 (phospho S539)
- Suitable for: WB
- Reacts with: Mouse, Rat, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-EIF2B5 (phospho S539) antibody
EIF2B5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF2B5 (phospho S539) -
由来種
Rabbit -
特異性
This ab reacts with both human and rat (see, for example, PMID 21165642, 20484009 and 21832246) but it is not very clear if this recognition happens only when S535 (rat) and S544 (human) are phosphorylated or when S535 (rat)/S540 (human) are phosphorylated. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Rat EIF2B5 (phospho S535). Phosphopeptide derived from the region of rat eIF2Be that contains serine 535 (corresponding to serine 539 in the human sequence).
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ポジティブ･コントロール
- Recombinant eIF-2B epsilon treated with GSK 3 beta and lambda phosphatase, CHO-T cells transfected with human insulin receptor (IR) +/- insulin. L6, L6 differentiated to myotubes, L6 treated with Insulin (10nM for 10 min), C2C12 and C2C12 treated with Insulin (10nM for 10 min)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eukaryotic initiation factor 2B epsilon (eIF-2B epsilon), and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using an eIF-2B epsilon-derived peptide that is phosphorylated at serine 539. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4775の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 88 kDa (predicted molecular weight: 82 kDa).
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| 特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 88 kDa (predicted molecular weight: 82 kDa). |
ターゲット情報
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機能
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
関連疾患
Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. -
配列類似性
Belongs to the eIF-2B gamma/epsilon subunits family.
Contains 1 W2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 224045 Mouse
- Entrez Gene: 192234 Rat
- SwissProt: Q8CHW4 Mouse
- SwissProt: Q64350 Rat
- Unigene: 233855 Mouse
- Unigene: 10607 Rat
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別名
- CACH antibody
- CLE antibody
- EI2BE_HUMAN antibody
see all
画像
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Western blot - Anti-EIF2B5 (phospho S539) antibody (ab4775)Peptide Competition: Recombinant eIF-2B episilon treated with lambda phosphatase (1) or treated with GSK-3 beta (lanes 2-5) and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
µ g/mL ab4775 antibody, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phophoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4775 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the up-regulation of the ab4775 phospho signal upon stimulation with GSK-3 beta. Pep -
Western blot - Anti-EIF2B5 (phospho S539) antibody (ab4775)All lanes : Anti-EIF2B5 (phospho S539) antibody (ab4775) at 1/2000 dilution
Lane 1 : L6 whole cell extract
Lane 2 : L6 differentiated to myotubes
Lane 3 : L6 treated with Insulin (10nM for 10 min)
Lane 4 : C2C12
Lane 5 : C2C12 treated with Insulin (10nM for 10 min)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 82 kDaA 80 kDa band corresponding to Phospho-eIF2b epsilon was observed across the cell lines tested and reduced upon treatment and in differentiated cell line.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab4775 は 8 報の論文で使用されています。
- Lim JA et al. Therapeutic Benefit of Autophagy Modulation in Pompe Disease. Mol Ther 26:1783-1796 (2018). PubMed: 29804932
- Nichols K et al. Exogenous essential amino acids stimulate an adaptive unfolded protein response in the mammary glands of lactating cows. J Dairy Sci 100:5909-5921 (2017). PubMed: 28478014
- Bajramaj DL et al. Addition of glycerol to lactating cow diets stimulates dry matter intake and milk protein yield to a greater extent than addition of corn grain. J Dairy Sci 100:6139-6150 (2017). PubMed: 28601462
- Ayuso MI et al. Stress Granule Induction after Brain Ischemia Is Independent of Eukaryotic Translation Initiation Factor (eIF) 2a Phosphorylation and Is Correlated with a Decrease in eIF4B and eIF4E Proteins. J Biol Chem 291:27252-27264 (2016). PubMed: 27836976
- Doelman J et al. Essential amino acid infusions stimulate mammary expression of eukaryotic initiation factor 2Be but milk protein yield is not increased during an imbalance. J Dairy Sci 98:4499-508 (2015). PubMed: 25912861
- Doelman J et al. Branched-chain amino acid and lysine deficiencies exert different effects on mammary translational regulation. J Dairy Sci 98:7846-55 (2015). PubMed: 26342977
- Sidrauski C et al. Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2:e00498 (2013). WB ; Human . PubMed: 23741617
- Yung HW et al. Evidence of placental translation inhibition and endoplasmic reticulum stress in the etiology of human intrauterine growth restriction. Am J Pathol 173:451-62 (2008). WB ; Human . PubMed: 18583310
