Anti-EID1 抗体 [26] (ab78323)
![Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)](https://www.abcam.co.jp/ps/products/78/ab78323/Images/ab78323-220780-78323-IF.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)")
Key features and details
- Mouse monoclonal [26] to EID1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-EID1 antibody [26]
EID1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [26] to EID1 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide:
YEKTPFDQLAFIEELFSLMVVNRLTEELGC
, corresponding to amino acids 159 - 187 of Human EID1 -
ポジティブ･コントロール
- extracts from MCF7 cells transfected with EID1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
特記事項（精製）
ab78323 was purified by propriety chromatography under mild conditions as IgG fraction from serum free growth medium of mouse hybridoma clone and sterilized by filtration. -
ポリ/モノ
モノクローナル -
クローン名
26 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. |
ターゲット情報
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機能
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. -
組織特異性
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines. -
発生段階
Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). -
翻訳後修飾
Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. -
細胞内局在
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 23741 Human
- Omim: 605894 Human
- SwissProt: Q9Y6B2 Human
- Unigene: 255973 Human
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別名
- 21 kDa pRb associated protein antibody
- 21 kDa pRb-associated protein antibody
- C15orf3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)
ab78323 staining EID1 in HeLA cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 0.25% in PBS and blocked with 1.5% BSA for 30 minutes. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) overnight at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/1000 in PBS + 1% BSA) was used as the secondary antibody incubated for 1 hour. DAPI was used to stain the nuclear DNA.
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![Western blot - Anti-EID1 antibody [26] (ab78323)](https://www.abcam.co.jp/ps/products/78/ab78323/Images/EID1-Primary-antibodies-ab78323-7.JPG)
Western blot - Anti-EID1 antibody [26] (ab78323)All lanes : Anti-EID1 antibody [26] (ab78323) at 1 µg/ml
Lane 1 : extracts from MCF7 cells transfected with control
vector pCMV1
Lane 2 : extracts from MCF7 cells transfected with the EID1 expression
vector pcDNA3/EID1
Secondary
All lanes : HRP-conjugated mouse IgG
Predicted band size: 21 kDa
Observed band size: 21 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab78323 は論文での使用が確認できていません。