Anti-EI2BL/MRI1 抗体 (ab187894)
Key features and details
- Rabbit polyclonal to EI2BL/MRI1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EI2BL/MRI1 antibody -
製品の詳細
Rabbit polyclonal to EI2BL/MRI1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Cynomolgus monkey
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免疫原
Recombinant fragment (proprietary-tag) corresponding to Human EI2BL/MRI1 aa 250 to the C-terminus.
Database link: Q9BV20 -
ポジティブ･コントロール
- Human thyroid gland tissue; RT-4, U-251MG sp cell lysates; U-2 OS cell line
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187894の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover. -
パスウェイ
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6. -
配列類似性
Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily. -
細胞内局在
Nucleus. Cytoplasm. Cell projection. Primarily nuclear, but cytoplasmic in cancer cells, with enrichment at leading edge of the plasma membrane in late stage tumor cells. - Information by UniProt
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参照データベース
- Entrez Gene: 534734 Cow
- Entrez Gene: 84245 Human
- Entrez Gene: 288912 Rat
- Omim: 615105 Human
- SwissProt: Q2NL31 Cow
- SwissProt: Q9BV20 Human
- SwissProt: Q5HZE4 Rat
- Unigene: 439370 Human
see all -
別名
- EI2BL antibody
- M1Pi antibody
- Mediator of RhoA-dependent invasion antibody
see all
画像
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Western blot - Anti-EI2BL/MRI1 antibody (ab187894)All lanes : Anti-EI2BL/MRI1 antibody (ab187894) at 1/1000 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251MG sp cell lysate -
Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (ab187894)Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EI2BL/MRI1 antibody (ab187894)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human thyroid gland tssue labeling EI2BL/MRI1 with ab187894 at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (ab187894)Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab187894 は 1 報の論文で使用されています。
- Li HR et al. Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype. J Clin Pathol 73:139-146 (2020). PubMed: 31586937