Anti-EHMT1/GLP 抗体 [B0422] (ab41969)
Key features and details
- Mouse monoclonal [B0422] to EHMT1/GLP
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-EHMT1/GLP antibody [B0422]
EHMT1/GLP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B0422] to EHMT1/GLP -
由来種
Mouse -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment corresponding to Mouse EHMT1/GLP aa 134-234.
Database link: Q5DW34 -
ポジティブ・コントロール
- WT mouse ES cell
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特記事項
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
B0422 -
ミエローマ
NS1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41969の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (2) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. -
組織特異性
Widely expressed. -
関連疾患
Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. -
配列類似性
Belongs to the histone-lysine methyltransferase family.
Contains 8 ANK repeats.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
ドメイン
The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
The SET domain mediates interaction with WIZ. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Chromosome. Associates with euchromatic regions. - Information by UniProt
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参照データベース
- Entrez Gene: 77683 Mouse
- SwissProt: Q5DW34 Mouse
- Unigene: 24176 Mouse
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別名
- bA188C12.1 antibody
- DKFZp667M072 antibody
- EHMT 1 antibody
see all
画像
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Ab41969 at 5 µg/ml staining WT mouse ES cell (Positive).
This image was generated using the ascites version of the product.
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Ab41969 at 5µg/ml staining GLP KO mouse ES cell (Negative).
This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab41969 は 29 報の論文で使用されています。
- Besschetnova A et al. Demethylation of EHMT1/GLP Protein Reprograms Its Transcriptional Activity and Promotes Prostate Cancer Progression. Cancer Res Commun 3:1716-1730 (2023). PubMed: 37663929
- Li M et al. LSD1 Inhibition Disrupts Super-Enhancer-Driven Oncogenic Transcriptional Programs in Castration-Resistant Prostate Cancer. Cancer Res 83:1684-1698 (2023). PubMed: 36877164
- Swaminathan J et al. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis. Front Oncol 12:855167 (2022). PubMed: 35600406
- Meng TG et al. Maternal EHMT2 is essential for homologous chromosome segregation by regulating Cyclin B3 transcription in oocyte meiosis. Int J Biol Sci 18:4513-4531 (2022). PubMed: 35864958
- Vinson DA et al. De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a. Epigenetics Chromatin 15:36 (2022). PubMed: 36411491