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リコンビナントRabMAb

Anti-EHD2 抗体 [EPR9821] (ab154784)

Submit a review Submit a question References (2)
Western blot - Anti-EHD2 antibody [EPR9821] (ab154784)
  • Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (ab154784)
  • Anti-EHD2 antibody [EPR9821] (ab154784)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9821] to EHD2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-EHD2 antibody [EPR9821]
    EHD2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9821] to EHD2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Fetal heart, A549 and HeLa lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9821

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154784の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
ICC/IF
1/250 - 1/500.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
ICC/IF
1/250 - 1/500.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

  • 組織特異性

    Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.

  • 配列類似性

    Contains 1 EF-hand domain.
    Contains 1 EH domain.

  • ドメイン

    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

  • 細胞内局在

    Cell membrane. Endosome membrane. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EH domain containing 2 antibody
    • EH domain containing 3 antibody
    • EH domain containing protein 2 antibody
    • EH domain-containing protein 2 antibody
    • EHD 2 antibody
    • Ehd2 antibody
    • EHD2_HUMAN antibody
    • EHD3 antibody
    • FLJ96617 antibody
    • PAST 2 antibody
    • PAST homolog 2 antibody
    • PAST2 antibody
    see all

画像

  • Western blot - Anti-EHD2 antibody [EPR9821] (ab154784)
    Western blot - Anti-EHD2 antibody [EPR9821] (ab154784)
    All lanes : Anti-EHD2 antibody [EPR9821] (ab154784) at 1/1000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (ab154784)
    Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (ab154784)
    Immunofluorescent analysis of HeLa cells labeling EHD2 with ab154784 at 1/250 dilution.
  • Anti-EHD2 antibody [EPR9821] (ab154784)
    Anti-EHD2 antibody [EPR9821] (ab154784)

参考文献 (2)

ab154784 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154784 は 2 報の論文で使用されています。

  • Morén B  et al. EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes. Mol Biol Cell 30:1147-1159 (2019). PubMed: 30811273
  • Li Z  et al. A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Sci Adv 2:e1601470 (2016). PubMed: 28028537

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