Anti-EHD2 抗体

• ICC

Supplier Data

Immunocytochemistry - Anti-EHD2 antibody (AB23935)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

• ICC

Supplier Data

Immunocytochemistry - Anti-EHD2 antibody (AB23935)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

• WB

Supplier Data

Western blot - Anti-EHD2 antibody (AB23935)

Lysate in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-EHD2 antibody (ab23935) at 0.1 µg/mL

Lane 1:

Human Lung tissue lysate at 35 µg

Lane 2:

Human colon tissue lysate at 35 µg

Lane 3:

Human placenta tissue lysate at 35 µg

false

• ICC

Unknown

Immunocytochemistry - Anti-EHD2 antibody (AB23935)

ICC/IF image of ab23935 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23935, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EHD2 antibody (AB23935)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.