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リコンビナントRabMAb

Anti-EGR2 抗体 [EPR23228-40] (ab245228)

Submit a review Submit a question References (2)
Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Flow Cytometry (Intracellular) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
  • Anti-EGR2 antibody [EPR23228-40] (ab245228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23228-40] to EGR2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE

製品の概要

  • 製品名

    Anti-EGR2 antibody [EPR23228-40]
    EGR2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23228-40] to EGR2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: ChIP,ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse P0 cerebellum and Mouse thymus tissue lysates; Rat thymus and Rat brain lysates. IHC-P: Mouse cerebrum tissue; Rat cerebrum and Rat skin tissue. Flow Cyt (intra): Mouse splenocytes treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245228の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ChIP,ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.

  • 関連疾患

    Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN) [MIM:605253]. Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot-Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities.
    Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D) [MIM:607678]. CMT1D is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
    Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.

  • 配列類似性

    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.

  • 翻訳後修飾

    Ubiquitinated by WWP2 leading to proteasomal degradation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AT591 antibody
      • CMT1D antibody
      • CMT4E antibody
      • DKFZp686J1957 antibody
      • E3 SUMO-protein ligase EGR2 antibody
      • Early growth response 2 antibody
      • Early growth response protein 2 antibody
      • EGR-2 antibody
      • egr2 antibody
      • EGR2_HUMAN antibody
      • FLJ14547 antibody
      • KROX 20 Drosophila homolog antibody
      • Krox 20 homolog Drosophila antibody
      • KROX-20, Drosophila, homolog (early growth response-2) antibody
      • KROX20 antibody
      • Krox20 protein antibody
      • Zinc finger protein Krox-20 antibody
      see all

    画像

    • Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      All lanes : Anti-EGR2 antibody [EPR23228-40] (ab245228) at 1/1000 dilution

      Lane 1 : Mouse P0 cerebellum tissue lysate
      Lane 2 : Mouse thymus tissue lysate
      Lane 3 : Rat thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 50 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 23747308).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

      Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat skin (PMID: 21514423, 21514423).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Flow Cytometry (Intracellular) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Flow Cytometry (Intracellular) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

      Intracellular flow cytometric analysis of 2% paraformaldehyde fixed, True-Nuclear buffer-permeabilized Mouse splenocyte (treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h) cells labelling EGR2 with ab245228 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      Cells were surface stained with anti-CD4 conjugated to APC-Cy7 . Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or ab245228 (Right). 

       

       

    • Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Anti-EGR2 antibody [EPR23228-40] (ab245228) at 1/1000 dilution + Rat brain tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 50 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Expopsure time: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 23747308).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat cerebrum.The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the mouse cerebrum (PMID: 30122531).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Anti-EGR2 antibody [EPR23228-40] (ab245228)
      Anti-EGR2 antibody [EPR23228-40] (ab245228)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab245228 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245228 は 2 報の論文で使用されています。

    • Bo Z  et al. EGR2 is a hub-gene in myocardial infarction and aggravates inflammation and apoptosis in hypoxia-induced cardiomyocytes. BMC Cardiovasc Disord 22:373 (2022). PubMed: 35971091
    • Dolicka D  et al. Tristetraprolin Promotes Hepatic Inflammation and Tumor Initiation but Restrains Cancer Progression to Malignancy. Cell Mol Gastroenterol Hepatol 11:597-621 (2021). PubMed: 32987153

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