Anti-EGFR (phospho Y992) 抗体 [EM-12] (ab81440)
![Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.co.jp/ps/products/81/ab81440/Images/ab81440-514424-anti-egfr-phospho-y992-antibody-em-12-flow-cytometry-a431-cells-human.jpg "Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)")
Key features and details
- Mouse monoclonal [EM-12] to EGFR (phospho Y992)
- Suitable for: WB, IP, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-EGFR (phospho Y992) antibody [EM-12]
EGFR 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EM-12] to EGFR (phospho Y992) -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human EGFR (phospho Y992).
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ポジティブ･コントロール
- EGF-stimulated A431. A431, CALU-3, Jurkat cell lines. Flow cyto(Intra): A431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant. Purity: > 95% (by SDS-PAGE). -
ポリ/モノ
モノクローナル -
クローン名
EM-12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa).
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa). |
|
IP
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. |
ターゲット情報
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機能
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
組織特異性
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
関連疾患
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
細胞内局在
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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参照データベース
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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別名
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)
Separation of EGF stimulated A431 cell suspension stained using ab81440 at 1μg/ml (red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).
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![Western blot - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.co.jp/ps/products/81/ab81440/Images/ab81440-96627-anti-egfr-phospho-y992-antibody-em-12-western-blot.jpg)
Western blot - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)All lanes : Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440) at 1 µg/ml
Lane 1 : EGF-treated A431 cell line
Lane 2 : EGF-treated CALU-3 cell line
Lane 3 : EGF-treated MCF-7 cell line
Lane 4 : EGF-treated Jurkat cell line
Lane 5 : EGF-treated Ramos cell line
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.co.jp/ps/products/81/ab81440/Images/ab81440-96626-anti-egfr-phospho-y992-antibody-em-12-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with ab81440 at 1µg/ml and goat anti-mouse-HRP. -
![Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.co.jp/ps/products/81/ab81440/Images/ab81440-96625-anti-egfr-phospho-y992-antibody-em-12-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab81440 は 6 報の論文で使用されています。
- Deng QF et al. Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis. J Cancer 11:3667-3674 (2020). PubMed: 32284763
- Hu DD et al. SKA3 promotes lung adenocarcinoma metastasis through the EGFR-PI3K-Akt axis. Biosci Rep 40:N/A (2020). PubMed: 32068236
- Pei YF et al. Silencing of LAMC2 Reverses Epithelial-Mesenchymal Transition and Inhibits Angiogenesis in Cholangiocarcinoma via Inactivation of the Epidermal Growth Factor Receptor Signaling Pathway. Am J Pathol 189:1637-1653 (2019). PubMed: 31345467
- Dittmann K et al. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. PLoS One 12:e0189087 (2017). PubMed: 29253018
- Needham SR et al. EGFR oligomerization organizes kinase-active dimers into competent signalling platforms. Nat Commun 7:13307 (2016). PubMed: 27796308
- Harrison H et al. Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Res 15:R21 (2013). WB ; Human . PubMed: 23497505