Anti-EGFR (phospho Y1173) 抗体 [E124] (ab32578)
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.co.jp/ps/products/32/ab32578/Images/ab32578-478748-anti-egfr-phospho-y1173-antibody-e124-westernblot-A431-human.jpg "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E124] to EGFR (phospho Y1173)
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EGFR (phospho Y1173) antibody [E124]
EGFR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E124] to EGFR (phospho Y1173) -
由来種
Rabbit -
特異性
The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor).
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アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra), WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): Trichostatin A treated HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E124 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
- Alexa Fluor® 594 Anti-EGFR (phospho Y1173) antibody [E124] (ab207020)
- Alexa Fluor® 405 Anti-EGFR (phospho Y1173) antibody [E124] (ab207235)
- Alexa Fluor® 555 Anti-EGFR (phospho Y1173) antibody [E124] (ab207236)
- Alexa Fluor® 488 Anti-EGFR (phospho Y1173) antibody [E124] (ab207345)
- Alexa Fluor® 647 Anti-EGFR (phospho Y1173) antibody [E124] (ab207346)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/25 - 1/50.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).
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| 特記事項 |
|---|
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ICC/IF
1/25 - 1/50. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). |
ターゲット情報
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機能
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
組織特異性
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
関連疾患
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
細胞内局在
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
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参照データベース
- Entrez Gene: 1956 Human
- Omim: 131550 Human
- SwissProt: P00533 Human
- Unigene: 488293 Human
- Unigene: 605083 Human
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別名
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
画像
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Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)All lanes : Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution (Purified)
Lane 1 : Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 170 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.co.jp/ps/products/32/ab32578/Images/ab32578-9-anti-egfr-phospho-y1173-antibody-e124-immunocytochemistry-a431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with EGF(100ng/ml 5min) cells labeling EGFR with Purified ab32578 at 1:2000 dilution (0.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.co.jp/ps/products/32/ab32578/Images/ab32578-273112-anti-egfr-phospho-y1173-antibody-e124-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177178 at 1/1300 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)](https://www.abcam.co.jp/ps/products/32/ab32578/Images/ab32578-7-benefits-of-recombinant-antibodies.png)
Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab32578 は 35 報の論文で使用されています。
- Posey TA et al. Loss of LGR5 through Therapy-induced Downregulation or Gene Ablation Is Associated with Resistance and Enhanced MET-STAT3 Signaling in Colorectal Cancer Cells. Mol Cancer Ther 22:667-678 (2023). PubMed: 36921315
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177
- Zha Y et al. ADAMTS8 Promotes Cardiac Fibrosis Partly Through Activating EGFR Dependent Pathway. Front Cardiovasc Med 9:797137 (2022). PubMed: 35224040
- Elamin YY et al. Poziotinib for EGFR exon 20-mutant NSCLC: Clinical efficacy, resistance mechanisms, and impact of insertion location on drug sensitivity. Cancer Cell 40:754-767.e6 (2022). PubMed: 35820397
- Wahjudi LW et al. Integrating proteomics into precision oncology. Int J Cancer 148:1438-1451 (2021). PubMed: 32949162