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リコンビナントRabMAb

Anti-EGFR (phospho Y1068) 抗体 [Y38] - BSA and Azide free (ab171022)

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Immunoprecipitation - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)
  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)
  • Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y38] to EGFR (phospho Y1068) - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free
    EGFR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y38] to EGFR (phospho Y1068) - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IPmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human
    交差が予測される動物種: Pig

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab171022 is the carrier-free version of ab32430.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab171022の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 135 kDa.

Can be blocked with ab205609.
IP
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 135 kDa.

Can be blocked with ab205609.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.

  • 組織特異性

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

  • 関連疾患

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

  • 細胞内局在

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt

  • 参照データベース

    • 別名

      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    画像

    • Immunoprecipitation - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)
      Immunoprecipitation - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)

      ab32430 (purified) at 1/30 dilution (2ug) immunoprecipitating EGFR in A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug . A431 (Human epidermoid carcinoma epithelial cell) treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
      Lane 2 (+): ab32430 & A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32430 in A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32430).

    • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)
      Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)

      Immunocytochemistry/Immunofluorescence analysis of untreated, EGF treated and EGF + LP treated A431 cells labelling EGFR (phospho Y1068) with purified ab32430 (left) and EGFR with ab52894 (right) both at a dilution of 1/1000.

      Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      The image shows increased membrane staining after EGF (100ng/ml, 30min) treatment on A431 cells. The LP treatment decreased the increased membrane staining caused by EGF.

      ab52894 was used as a Pan control for ab32430. The results showed membrane and cytoplasmic staining on untreated, EGF and EGF + LP treated A431 cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32430).

    • Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)
      Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free (ab171022)

    参考文献 (0)

    ab171022 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab171022 は論文での使用が確認できていません。

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