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AB76300

Anti-EGFR (phospho S1046 + S1047) 抗体 [EP2259Y]

Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal EGFR phospho S1046 + S1047 antibody. Suitable for IP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 3 publications.

別名を表示する

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)

Immunocytochemistry/Immunofluorescence analysis of untreated and EGF (100ng/mL for 10 minutes) treated A431 cells labelling EGFR (phospho S1046 + S1047) with ab76300 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin (1/200). Nuclei counterstained with DAPI (blue).

Control 1 : untreated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.
Control 2 : EGF treated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.

Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)
  • IP

Lab

Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)

Purified ab76300 at 1/40 dilution (2μg) immunoprecipitating EGFR in A431 treated with EGF whole cell lysate.
Lane 1 (input) : A431 (Human epidermoid carcinoma epithelial cell) treated with EGF whole cell lysate 10μg
Lane 2 (+) : ab76300 + A431 treated with EGF whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76300 in A431 treated with EGF whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 180 kDa

All lanes:

Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)

Predicted band size: 134 kDa

false

Western blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)
  • WB

Unknown

Western blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)

All lanes:

Western blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300) at 1/1000000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

A431 cell lysate treated with EGF at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 134 kDa

Observed band size: 180 kDa

false

Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)
  • Dot

Unknown

Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (AB76300)

Primary antibody : ab76300 at a dilution of 1/1000.

Secondary antibody : Peroxidase conjugated-goat anti-rabbit IgG, (H+L) at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST

Lane 1 : EGFR (pS1046/pS1047) phospho peptide.
Lane 2 : EGFR non-phospho peptide.

Exposure time : 3 minutes.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2259Y

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, IP, WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Recognises EGFR phosphorylated on Serine 1046 and Serine 1047 of the mature human isoform 1 (corresponding to S1070 and S1071 from the precursor form P00533-1/p170)

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代替製品

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AB76153

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組合せ製品

Primary Antibodies

AB40815

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Primary Antibodies

AB32086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500000 - 1/1000000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR pS1046 + S1047
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadi1328 PubMed37756411

2023

Piezo1 activates noncanonical EGFR endocytosis and signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Pardo-Pastor,Jody Rosenblatt

Hepatology communications 7: PubMed37486965

2023

Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zengwei Tang,Yuan Yang,Wen Chen,Tingbo Liang

Cancer research 71:3268-77 PubMed21398406

2011

The IL-8-regulated chemokine receptor CXCR7 stimulates EGFR signaling to promote prostate cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rajendra Kumar Singh,Bal L Lokeshwar
View all publications
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Abcam product promise

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