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リコンビナントRabMAb

Anti-EGF 抗体 [RM1015] (ab288294)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Western blot - Anti-EGF antibody [RM1015] (ab288294)
  • Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Western blot - Anti-EGF antibody [RM1015] (ab288294)
  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
  • Anti-EGF antibody [RM1015] (ab288294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1015] to EGF
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-EGF antibody [RM1015]
    EGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit recombinant multiclonal [RM1015] to EGF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Fr, IPmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse kidney tissue lysate; rat kidney tissue lysate. IHC-P: Mouse kidney tissue; rat kidney tissue. IHC-Fr: Mouse kidney tissue; rat kidney tissue. IP: Mouse kidney tissue lysate; rat salivary gland tissue lysate.

  • 特記事項

    This product is a recombinant multiclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab288294の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 133 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.
IP
1/30.
特記事項
WB
1/1000. Predicted molecular weight: 133 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.
IP
1/30.

ターゲット情報

  • 機能

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

  • 組織特異性

    Expressed in kidney, salivary gland, cerebrum and prostate.

  • 関連疾患

    Hypomagnesemia 4

  • 配列類似性

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.

  • 翻訳後修飾

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Beta urogastrone antibody
      • beta-urogastrone antibody
      • EGF antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling EGF with ab288294 at 1/1000 (0.5 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on the distal convoluted tubules in mouse kidney. The section was incubated with ab288294 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling EGF with ab288294 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in the renal tubule on the mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. 

    • Western blot - Anti-EGF antibody [RM1015] (ab288294)
      Western blot - Anti-EGF antibody [RM1015] (ab288294)
      All lanes : Anti-EGF antibody [RM1015] (ab288294) at 1/1000 dilution

      Lane 1 : Mouse kidney lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat kidney lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 160,45,56,66,97 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer and concentration are 5% NFDM/TBST. 

      Negative control: Mouse spleen and Rat spleen.

      The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2268351).

      Freshly made lysate is highly recommended to avoid EGF degradation and for better detection.

    • Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)
      Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)

      EGF was immunoprecipitated from 0.35 mg mouse kidney lysate with ab288294  at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using aab288294 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse kidney lysate 10µg

      Lane 2: ab288294 IP in Mouse kidney lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab288294 in Mouse kidney lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 23 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on the distal convoluted tubules in rat kidney. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Western blot - Anti-EGF antibody [RM1015] (ab288294)
      Western blot - Anti-EGF antibody [RM1015] (ab288294)
      All lanes : Anti-EGF antibody [RM1015] (ab288294) at 1/1000 dilution

      Lane 1 : Mouse kidney lysate
      Lane 2 : Rat salivary gland lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 160,45,56,66,97,6 kDa why is the actual band size different from the predicted?


      Exposure time: 100 seconds


      Blocking and diluting buffer and concentration are 5% NFDM/TBST. 

      The molecular weight observed is consistent with what has been described in the literature (PMID:1636692, 2260661, 7499272 & 2268351).

      Freshly made lysate is highly recommended to avoid EGF degradation and for better detection.

    • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney (fresh) tissue labeling EGF with ab288294 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in the renal tubule on the rat kidney is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. 

    • Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)
      Immunoprecipitation - Anti-EGF antibody [RM1015] (ab288294)

      EGF was immunoprecipitated from 0.35 mg rat salivary gland lysate with ab288294 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288294 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Rat salivary gland lysate 10µg

      Lane 2: ab288294 IP in Rat salivary gland lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab288294 in rat salivary gland lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 100 seconds. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on rat spleen. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] (ab288294)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling EGF with ab288294 at 1/1000 (0.5 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse spleen. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Anti-EGF antibody [RM1015] (ab288294)
      Anti-EGF antibody [RM1015] (ab288294)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab288294 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab288294 は論文での使用が確認できていません。

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