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リコンビナントRabMAb

Anti-EGF 抗体 [EPR19174] (ab184266)

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Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)
  • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)
  • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
  • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
  • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
  • Immunoprecipitation - Anti-EGF antibody [EPR19174] (ab184266)
  • Anti-EGF antibody [EPR19174] (ab184266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19174] to EGF
  • Suitable for: WB, IHC-Fr, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-EGF antibody [EPR19174]
    EGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19174] to EGF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Fr, IPmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse EGF recombinant protein; Mouse salivary gland and kidney lysates; Mouse urine sample. IHC-Fr: Mouse kidney tissue. IP: Mouse urine sample

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184266の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa).
IHC-Fr
1/500.
IP
1/40.
特記事項
WB
1/1000. Detects a band of approximately 160, 6 kDa (predicted molecular weight: 133 kDa).
IHC-Fr
1/500.
IP
1/40.

ターゲット情報

  • 機能

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

  • 組織特異性

    Expressed in kidney, salivary gland, cerebrum and prostate.

  • 関連疾患

    Hypomagnesemia 4

  • 配列類似性

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.

  • 翻訳後修飾

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Beta urogastrone antibody
      • beta-urogastrone antibody
      • EGF antibody
      • EGF_HUMAN antibody
      • Epidermal growth factor antibody
      • HOMG4 antibody
      • OTTHUMP00000219721 antibody
      • OTTHUMP00000219722 antibody
      • Pro epidermal growth factor antibody
      • URG antibody
      • Urogastrone antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)
      Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasmic staining on mouse kidney is observed. The staining pattern is consistent with what has been described in the literature (PMID:17671655). 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)
      Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR19174] (ab184266)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling EGF with ab184266 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

      Weak staining on mouse liver is observed. The expression profile observed is consistent with what has been described in the literature (PMID:17671655). 

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      Anti-EGF antibody [EPR19174] (ab184266) at 1/2000 dilution + Mouse EGF recombinant protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 6 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      Anti-EGF antibody [EPR19174] (ab184266) at 1/1000 dilution + Mouse salivary gland lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 6 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:2268351 & 7499272).

    • Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      Western blot - Anti-EGF antibody [EPR19174] (ab184266)
      All lanes : Anti-EGF antibody [EPR19174] (ab184266) at 1/1000 dilution

      Lane 1 : Mouse kidney lysate
      Lane 2 : Mouse urine sample

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 133 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 8 seconds; Lane 2: 5 seconds.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:2268351 & 7499272).

    • Immunoprecipitation - Anti-EGF antibody [EPR19174] (ab184266)
      Immunoprecipitation - Anti-EGF antibody [EPR19174] (ab184266)

      EGF was immunoprecipitated from 0.35 mg of Mouse urine sample with ab184266 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184266 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Mouse urine sample, 10µg (Input).

      Lane 2: ab184266 IP in Mouse urine sample.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184266 in Mouse urine sample.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-EGF antibody [EPR19174] (ab184266)
      Anti-EGF antibody [EPR19174] (ab184266)

    参考文献 (0)

    ab184266 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184266 は論文での使用が確認できていません。

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