Anti-EEF2K 抗体 [EP881Y] (ab45168)
![Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)](https://www.abcam.co.jp/ps/products/45/ab45168/Images/ab45168-260960-anti-eef2k-antibody-ep881y-western-blot.jpg "Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)")
Key features and details
- Rabbit monoclonal [EP881Y] to EEF2K
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EEF2K antibody [EP881Y]
EEF2K 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP881Y] to EEF2K -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human EEF2K aa 700-800 (C terminal). The exact sequence is proprietary.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP881Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45168の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 82 kDa.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 82 kDa. |
ターゲット情報
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機能
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
配列類似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
別名
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
画像
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Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)All lanes : Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution
All lanes :
Predicted band size: 82 kDaLane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab45168 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45168 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab45168 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)](https://www.abcam.co.jp/ps/products/45/ab45168/Images/ab45168-57329-anti-eef2k-antibody-ep881y-western-blot.jpg)
Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution + HEK293 cell lysate
Predicted band size: 82 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab45168 は 7 報の論文で使用されています。
- Aydemir E et al. Impact of silencing eEF2K expression on the malignant properties of chordoma. Mol Biol Rep 50:3011-3022 (2023). PubMed: 36652154
- Chen X et al. eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma. J Immunother Cancer 10:N/A (2022). PubMed: 35347072
- Suzuki K et al. Convergence of distinct signaling pathways on synaptic scaling to trigger rapid antidepressant action. Cell Rep 37:109918 (2021). PubMed: 34731624
- Feng T et al. Eef2k is not required for fertility in male mice. Transl Androl Urol 10:1988-1999 (2021). PubMed: 34159079
- Chen XS et al. UCH-L1-mediated Down-regulation of Estrogen Receptor a Contributes to Insensitivity to Endocrine Therapy for Breast Cancer. Theranostics 10:1833-1848 (2020). PubMed: 32042339
- Guan Y et al. Combined treatment of mitoxantrone sensitizes breast cancer cells to rapalogs through blocking eEF-2K-mediated activation of Akt and autophagy. Cell Death Dis 11:948 (2020). PubMed: 33144562
- Tavares CD et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem 289:23901-16 (2014). Human . PubMed: 25012662