Anti-EEF2K 抗体 (ab85721)
Key features and details
- Rabbit polyclonal to EEF2K
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EEF2K antibody
EEF2K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EEF2K -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human EEF2K, NP_037434.1
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85721の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 95 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
配列類似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 29904 Human
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: P70531 Rat
- Unigene: 498892 Human
- Unigene: 10958 Rat
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別名
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
画像
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Western blot - Anti-EEF2K antibody (ab85721)All lanes : Anti-EEF2K antibody (ab85721) at 0.1 µg/ml
Lane 1 : HeLa cells lysate at 50 µg
Lane 2 : HeLa cells lysate at 15 µg
Lane 3 : HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Additional bands at: 110 kDa, 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody (ab85721)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-EEF2K antibody (ab85721)Detection of Human EEF2K by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85721 at 3 ug/mg lysate. Subsequent Western blot detection was performed using 1 ug/ml ab85721. Detection: Chemiluminescence with exposure times of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85721 は 1 報の論文で使用されています。
- Chen X et al. eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma. J Immunother Cancer 10:N/A (2022). PubMed: 35347072