Anti-EEF2/Elongation factor 2 (phospho T56 + T58) 抗体 [EP833Y] (ab82981)
![Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981)](https://www.abcam.co.jp/ps/products/82/ab82981/Images/ab82981-97709-anti-eef2-phospho-t56-t58-antibody-ep833y-western-blot.jpg "Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP833Y] to EEF2/Elongation factor 2 (phospho T56 + T58)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y]
EEF2/Elongation factor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP833Y] to EEF2/Elongation factor 2 (phospho T56 + T58) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293 cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP833Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82981の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 95 kDa.
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| 特記事項 |
|---|
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WB
1/2000. Predicted molecular weight: 95 kDa. |
ターゲット情報
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機能
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. -
配列類似性
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
翻訳後修飾
Phosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1938 Human
- Entrez Gene: 13629 Mouse
- Entrez Gene: 29565 Rat
- Omim: 130610 Human
- SwissProt: P13639 Human
- SwissProt: P58252 Mouse
- SwissProt: P05197 Rat
- Unigene: 515070 Human
see all -
別名
- EEF 2 antibody
- Eef2 antibody
- EF-2 antibody
see all
画像
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Western blot - Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981)All lanes : Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilution
Lane 1 : Untreated 293 cell lysate
Lane 2 : Serum starved 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDa -
![Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981)](https://www.abcam.co.jp/ps/products/82/ab82981/Images/ab82981-2-benefits-of-recombinant-antibodies.png)
Anti-EEF2/Elongation factor 2 (phospho T56 + T58) antibody [EP833Y] (ab82981)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab82981 は 5 報の論文で使用されています。
- Xing L et al. Mutant neuropeptide S receptor reduces sleep duration with preserved memory consolidation. Sci Transl Med 11:N/A (2019). PubMed: 31619542
- Wang Z et al. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. Nature 558:435-439 (2018). PubMed: 29899451
- Tavares CD et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem 289:23901-16 (2014). Human . PubMed: 25012662
- Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol 589:3623-40 (2011). WB ; Mouse . PubMed: 21606113
- Nogueira da Costa A et al. An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol. Biochim Biophys Acta 1814:850-7 (2011). PubMed: 21513824