AB245412

Anti-EEF2/Elongation factor 2 抗体

Name: Anti-EEF2/Elongation factor 2 抗体 (ab245412) | Abcam
Brand: Abcam Limited
SKU: AB245412
Price: 90000 JPY
Availability: InStock

Anti-EEF2/Elongation factor 2 antibody

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Polyclonal EEF2/Elongation factor 2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human EEF2 aa 800 to C-terminus.

別名を表示する

EF2, EEF2, Elongation factor 2, EF-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody (AB245412)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody (AB245412)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for EEF2/Elongation factor 2 using ab245412 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Western blot - Anti-EEF2/Elongation factor 2 antibody (AB245412)

All lanes:

Western blot - Anti-EEF2/Elongation factor 2 antibody (ab245412) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa hole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 95 kDa

true

Exposure time: 1s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human EEF2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13639

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab245412 was affinity purified using an epitope specific to EEF2/Elongation factor 2 immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EEF2 or elongation factor 2 is an important protein in the process of translation during protein synthesis. It is also known by the names EF-2 and EF2. This protein facilitates the translocation step where it moves tRNA and mRNA from the A-site to the P-site on the ribosome. EEF2 has a molecular weight of approximately 95 kDa. The protein is ubiquitously expressed in various tissues and cells due to its fundamental role in protein synthesis making it essential for cellular activities.

Biological function summary

EEF2 functions as part of a larger ribosomal complex. It has an important role in ensuring the accuracy and efficiency of elongation during the translation process. This process is energy-dependent with EEF2 requiring GTP to catalyze the movement along the mRNA strand. The protein must undergo reversible phosphorylation which directly affects its activity. This post-translational modification of EEF2 is a significant modulation point influencing the rate of protein synthesis.

Pathways

EEF2 plays an integral role in the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway EEF2 activity is regulated to control protein translation based on cellular energy status and nutrient availability. This pathway is important for cell growth and proliferation. EEF2 interacts with other proteins such as factor 2 and eEF2kinase which phosphorylates the protein thereby inhibiting its activity. In the insulin signaling pathway similar regulatory mechanisms impact EEF2’s role in translation.

EEF2 connects to cancer and neurodegenerative diseases. In cancer aberrant regulation of EEF2 can lead to uncontrolled protein synthesis promoting cell proliferation and tumor growth. Its interaction with mTOR and other signaling proteins like ribosomal proteins plays a role in this dysregulation. In neurodegenerative conditions such as Alzheimer’s disease impaired EEF2 activity results in decreased synaptic protein synthesis affecting neuronal function. Understanding EEF2’s regulation and dysregulation provides insight into its involvement in these diseases offering potential for therapeutic intervention.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed : 26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed : 26593721).

See full target information EEF2

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEF2/Elongation factor 2 抗体

Anti-EEF2/Elongation factor 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody (AB245412)

Western blot - Anti-EEF2/Elongation factor 2 antibody (AB245412)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise