AB228642

Anti-eEF1B2 抗体

Anti-eEF1B2 antibody

JP Deleterious:医薬用外劇物

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(3 Publications)

Rabbit Polyclonal eEF1B2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1B2.

別名を表示する

EEF1B, EF1B, EEF1B2, Elongation factor 1-beta, EF-1-beta

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-eEF1B2 antibody (AB228642)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eEF1B2 antibody (AB228642)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for eEF1B2 (green) using ab228642 at 1/200 dilution in ICC/IF.

Nuclear counterstain : DNA probe (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (AB228642)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (AB228642)

Paraffin-embedded U87 xenograft tissue stained for eEF1B2 using ab228642 at 1/250 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (AB228642)

Paraffin-embedded mouse duodenum tissue stained for eEF1B2 using ab228642 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-eEF1B2 antibody (AB228642)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-eEF1B2 antibody (ab228642) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-eEF1B2 antibody (AB228642)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-eEF1B2 antibody (ab228642) at 1/5000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 24 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human EEF1B2. The exact immunogen used to generate this antibody is proprietary information.

P24534

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation elongation factor 1 beta 2 (eEF1B2) also known as EF1B or EF1-beta plays a role in protein translation. Mechanically eEF1B2 is a subunit of the elongation factor complex that facilitates the exchange of GDP for GTP on eEF1A allowing it to deliver aminoacyl-tRNAs to the ribosome during the elongation phase of translation. The protein has an estimated mass of approximately 24 kDa. It expresses widely throughout different tissues indicating its involvement in general cellular processes.

Biological function summary

EEF1B2 assists in the regulation of protein synthesis by forming a complex with other subunits of the elongation factor including eEF1A and eEF1D. This complex ensures the efficient and accurate delivery of tRNA during the translational elongation phase which is essential for proper cellular function and growth. Although eEF1B2 is often expressed in various tissues its expression levels can vary potentially affecting the rate of protein synthesis in different cellular environments.

Pathways

EEF1B2 integrates into the larger translation pathway a primary component of the cellular protein expression network. It also participates in the amino acid and nucleotide biosynthesis pathways by assisting in creating critical cellular proteins. eEF1B2 closely interacts with eEF1A within these pathways demonstrating its direct involvement in translation and its influence on the production of proteins required for other metabolic processes.

The dysregulation of eEF1B2 expression correlates with certain cancers such as ovarian and colorectal cancers where its abnormal expression may disrupt normal protein synthesis and promote tumor growth. Research suggests that eEF1B2 may interact with oncogenic proteins such as c-Myc impacting cell proliferation and survival mechanisms. Understanding the regulation and function of eEF1B2 in these contexts remains vital for developing targeted therapeutic strategies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

See full target information EEF1B2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging 6:1621808 PubMed40636718

2025

The lifespan-extending MEK1 inhibitor trametinib promotes regulation of de novo lipogenesis enzymes by chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexian Chen,Joshua Berg,Calvin M Burns,Hanyi Jia,Xinna Li,Richard A Miller,S Joseph Endicott,Gonzalo Garcia

iScience 27:109997 PubMed38868177

2024

Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bai,Yiqing You,Daoxun Chen,Yongmei Chen,Zhenjie Yin,Shangfan Liao,Bingyong You,Dongming Lu,Yingming Sun,Lixian Wu,Yongyang Wu

The Journal of cell biology 222: PubMed37418003

2023

PTEN is both an activator and a substrate of chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine K Zhang,Calvin M Burns,Mary E Skinner,David B Lombard,Richard A Miller,S Joseph Endicott

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eEF1B2 抗体

Anti-eEF1B2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-eEF1B2 antibody (AB228642)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (AB228642)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1B2 antibody (AB228642)

Western blot - Anti-eEF1B2 antibody (AB228642)

Western blot - Anti-eEF1B2 antibody (AB228642)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise