Anti-eEF1A1+eEF1A2+eEF1AL3 抗体 (ab37969)
Key features and details
- Rabbit polyclonal to eEF1A1+eEF1A2+eEF1AL3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-eEF1A1+eEF1A2+eEF1AL3 antibody -
製品の詳細
Rabbit polyclonal to eEF1A1+eEF1A2+eEF1AL3 -
由来種
Rabbit -
特異性
Due to sequence homology the antibody recognises all three eEF1A forms, eEF1A1, eEF1A2 and eEF1A3. Through WB ab37969 was found to detect recombinant and endogenous eF1A1 and eF1A2. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human eEF1A1+eEF1A2+eEF1AL3 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37968) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following: Human Whole Cell Lysates (HeLa, Jurkat, A431, HEK 293, HepG2, MCF-7, SHSY-5Y, U2OS) Mouse Whole Cell Lysates (NIH 3T3, MEF1) Mouse Tissue Lysates (Brain, Liver, Heart, Kidney, Pancreas, Testis, Skeletal muscle, Spinal Cord, Ovary) Rat Whole Cell Lysate(PC12) Rat Tissue Lysate (Brain, Liver, Heart)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37969の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
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関連性
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Tissue specificity eEF1A1: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. Tissue specificity eEF1A2: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. PTM eEF1A1: ISGylated. -
参照データベース
- SwissProt: P68104 Human
- SwissProt: Q05639 Human
- SwissProt: Q5VTE0 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
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別名
- CCS3 antibody
- eEF1A-1 antibody
- eEF1A-2 antibody
see all
画像
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All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa -
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 :Recombinant Human eEF1A1/EF-Tu protein (ab81792)
Lane 2 : Human EEF1A2 full length protein (ab40317)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab37969 は 11 報の論文で使用されています。
- Rapone R et al. The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells. iScience 26:107386 (2023). PubMed: 37559904
- Endicott SJ et al. Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy. Autophagy 17:612-625 (2021). PubMed: 32013718
- Wu M et al. URGCP/URG4 promotes apoptotic resistance in bladder cancer cells by activating NF-?B signaling. Oncotarget 6:30887-901 (2015). PubMed: 26429874
- Dong C et al. Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. J Neurosci 34:3171-82 (2014). PubMed: 24573276
- Häsler J et al. Cytoplasmic activation-induced cytidine deaminase (AID) exists in stoichiometric complex with translation elongation factor 1a (eEF1A). Proc Natl Acad Sci U S A 108:18366-71 (2011). PubMed: 22042842