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AB4469

Anti-EED 抗体

Anti-EED antibody

5

(3 Reviews)

|

(32 Publications)

Rabbit Polyclonal EED antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 32 publications.

別名を表示する

Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EED antibody (AB4469)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EED antibody (AB4469)

Rabbit polyclonal to EED (ab4469) on U-2 OS (Human bone osteosarcoma epithelial cell line) cells. Antibody used at 1/100.

U-2 OS cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).

Secondary antibody : donkey anti-rabbit (Cy3).

Western blot - Anti-EED antibody (AB4469)
  • WB

Lab

Western blot - Anti-EED antibody (AB4469)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : EED knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab4469 was shown to recognize EED in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when EED knockout samples were examined. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EED antibody (ab4469)

Predicted band size: 50 kDa

false

Western blot - Anti-EED antibody (AB4469)
  • WB

Project4585****

Western blot - Anti-EED antibody (AB4469)

ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).

All lanes:

Western blot - Anti-EED antibody (ab4469) at 1/250 dilution

All lanes:

EED Recombinant Protein (Human) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 80 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The EED protein also known as embryonic ectoderm development plays an important mechanical role within the cell. It is a component of the polycomb repressive complex 2 (PRC2) that mediates transcriptional silencing of target genes. The molecular weight of EED protein is approximately 50 kDa. EED is expressed in various tissues and is a critical mediator in maintaining transcriptional repression necessary for embryonic stem cell identity and differentiation.
Biological function summary

EED functions as a repressor of gene transcription through the polycomb group complex. It interacts with other components of PRC2 such as EZH2 SUZ12 to establish and maintain chromatin modifications. EED acts as a scaffold allowing communication between other proteins essential in the assembly and function of PRC2. These interactions regulate histone methylation contributing to gene silencing.

Pathways

EED plays a central role in the regulation of the Wnt and Hedgehog pathways. These pathways are critical for controlling cell fate decisions and developmental processes. In the Wnt pathway EED in conjunction with other PRC2 members modulates β-catenin-mediated transcription. In the Hedgehog pathway EED impacts the Gli family of transcription factors underlining its importance in development and cellular proliferation regulation.

EED has been linked to various cancers and developmental disorders. Aberrant EED function and localization relate to overactivation of the Wnt pathway an aspect seen in colorectal cancer. EED also interacts with EHZ2 contributing to tumorigenesis through unchecked cell division and proliferation. In the context of Weaver syndrome mutations within EED are involved consolidating its important association with developmental anomalies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
See full target information EED
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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111363 PubMed36130486

2022

Single-cell sequencing reveals activation of core transcription factors in PRC2-deficient malignant peripheral nerve sheath tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyuan Zhang,Hannah E Lou,Vishaka Gopalan,Zhihui Liu,Hilda M Jafarah,Haiyan Lei,Paige Jones,Carly M Sayers,Marielle E Yohe,Prashant Chittiboina,Brigitte C Widemann,Carol J Thiele,Michael C Kelly,Sridhar Hannenhalli,Jack F Shern

iScience 25:104827 PubMed35992062

2022

EZH2 T367 phosphorylation activates p38 signaling through lysine methylation to promote breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Gonzalez,Giuseppina Daniela Naimo,Talha Anwar,Alessandro Paolì,Shilpa R Tekula,Suny Kim,Natasha Medhora,Shoshana A Leflein,Jacob Itkin,Raymond Trievel,Kelley M Kidwell,Yu-Chih Chen,Loredana Mauro,Euisik Yoon,Sebastiano Andò,Celina G Kleer

Scientific reports 12:3655 PubMed35256668

2022

Link between the EZH2 noncanonical pathway and microtubule organization center polarization during early T lymphopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Frederique Deshayes,Magali Fradet,Sandra Kaminski,Mireille Viguier,Jean-Pol Frippiat,Stephanie Ghislin

Life science alliance 4: PubMed33361335

2020

The chromatin landscape of primary synovial sarcoma organoids is linked to specific epigenetic mechanisms and dependencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gaylor Boulay,Luisa Cironi,Sara P Garcia,Shruthi Rengarajan,Yu-Hang Xing,Lukuo Lee,Mary E Awad,Beverly Naigles,Sowmya Iyer,Liliane C Broye,Tugba Keskin,Alexandra Cauderay,Carlo Fusco,Igor Letovanec,Ivan Chebib,Petur Gunnalugur Nielsen,Stéphane Tercier,Stéphane Cherix,Tu Nguyen-Ngoc,Gregory Cote,Edwin Choy,Paolo Provero,Mario L Suvà,Miguel N Rivera,Ivan Stamenkovic,Nicolò Riggi

Cell death & disease 11:1061 PubMed33311453

2020

Inhibition of EZH2 enhances the therapeutic effect of 5-FU via PUMA upregulation in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Tan,Zhongqiang Zhang,Ping Liu,Hongliang Yao,Liangfang Shen,Jing-Shan Tong

Development (Cambridge, England) 147: PubMed32376680

2020

RNF220 is required for cerebellum development and regulates medulloblastoma progression through epigenetic modulation of Shh signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Ma,Tao An,Liang Zhu,Longlong Zhang,Huishan Wang,Biyu Ren,Bin Sun,Xia Zhou,Yan Li,Bingyu Mao

Genes, chromosomes & cancer 59:472-483 PubMed32259323

2020

Novel renal medullary carcinoma cell lines, UOK353 and UOK360, provide preclinical tools to identify new therapeutic treatments.

Applications

Unspecified application

Species

Unspecified reactive species

Darmood Wei,Youfeng Yang,Christopher J Ricketts,Cathy D Vocke,Mark W Ball,Carole Sourbier,Darawalee Wangsa,Danny Wangsa,Rajarshi Guha,Xiaohu Zhang,Kelli Wilson,Lu Chen,Paul S Meltzer,Thomas Ried,Craig J Thomas,Maria J Merino,W Marston Linehan

Nature genetics 52:264-272 PubMed32094912

2020

Chromatin interaction analyses elucidate the roles of PRC2-bound silencers in mouse development.

Applications

Unspecified application

Species

Unspecified reactive species

Chew Yee Ngan,Chee Hong Wong,Harianto Tjong,Wenbo Wang,Rachel L Goldfeder,Cindy Choi,Hao He,Liang Gong,Junyan Lin,Barbara Urban,Julianna Chow,Meihong Li,Joanne Lim,Vivek Philip,Stephen A Murray,Haoyi Wang,Chia-Lin Wei

Cell death & disease 10:685 PubMed31527584

2019

PICOT binding to chromatin-associated EED negatively regulates cyclin D2 expression by increasing H3K27me3 at the CCND2 gene promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Pinakin Pandya,Minesh Jethva,Eitan Rubin,Ramon Y Birnbaum,Alex Braiman,Noah Isakov

Nature communications 10:1897 PubMed31015486

2019

RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction-point.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Won Lee,Da-Mi Kim,Ju-Won Jang,Tae-Geun Park,Soo-Hyun Song,You-Soub Lee,Xin-Zi Chi,Il Yeong Park,Jin-Won Hyun,Yoshiaki Ito,Suk-Chul Bae
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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