Anti-EDJ 抗体 (ab75107)
Key features and details
- Rabbit polyclonal to EDJ
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-EDJ antibody
EDJ 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EDJ -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human EDJ aa 31-80 (internal sequence).
Database link: Q9UBS4 -
ポジティブ･コントロール
- IHC-P: human heart tissue. WB: extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75107の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 J domain. -
翻訳後修飾
Contains high-mannose Endo H-sensitive carbohydrates.
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven. -
細胞内局在
Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct. - Information by UniProt
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参照データベース
- Entrez Gene: 51726 Human
- Entrez Gene: 67838 Mouse
- Entrez Gene: 360734 Rat
- Omim: 611341 Human
- SwissProt: Q9UBS4 Human
- SwissProt: Q99KV1 Mouse
- SwissProt: Q6TUG0 Rat
- Unigene: 317192 Human
see all -
別名
- ABBP-2 antibody
- ABBP2 antibody
- APOBEC1 binding protein 2 antibody
see all
画像
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Western blot - Anti-EDJ antibody (ab75107)All lanes : Anti-EDJ antibody (ab75107) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HUVEC
cells
Lane 3 : extracts from COLO cells
Lane 4 : extracts from MCF-7 cells
Lane 5 : extracts from HeLa cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDJ antibody (ab75107)Immunohistochemical analysis of EDJ in paraffin embedded human heart tissue using 1/50 ab75107.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab75107 は 3 報の論文で使用されています。
- Cornec-Le Gall E et al. Monoallelic Mutations to DNAJB11 Cause Atypical Autosomal-Dominant Polycystic Kidney Disease. Am J Hum Genet 102:832-844 (2018). PubMed: 29706351
- Croner RS et al. Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis. Int J Cancer 135:2878-86 (2014). PubMed: 24771638
- Xu W et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). PubMed: 24198121