AB126622

Anti-EDG3/S1P3 抗体 [EPR4541(2)]

Anti-EDG3/S1P3 antibody [EPR4541(2)]

20ul selling size
RabMAb
Recombinant
詳細を見る

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal EDG3/S1P3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 11 publications.

別名を表示する

C9orf108, C9orf47, EDG3, S1PR3, Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

ab126622 at a 1/50 dilution staining EDG3/S1P3 in paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

All lanes:

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

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OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

OI-RD Scanning

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OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4541(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EDG3 also known as S1P3 is a G protein-coupled receptor with a molecular mass of approximately 43 kDa. It interacts with sphingosine-1-phosphate (S1P) a bioactive lipid molecule involved in diverse cellular processes. EDG3/S1P3 is expressed in various tissues such as the cardiovascular system lungs and lymphoid organs highlighting its importance in different physiological contexts. As a member of the S1P receptor family it plays a significant role in cellular communication.

Biological function summary

EDG3/S1P3 affects several critical processes such as cell proliferation survival and migration. It functions within complexes that modulate intracellular signaling pathways. The receptor is involved in the regulation of immune responses and vascular stability by mediating signals that maintain endothelial barrier function and lymphocyte trafficking. Its interactions with other S1P receptors also contribute to its broad influence on cellular functions.

Pathways

EDG3/S1P3 participates in the sphingolipid signaling pathways and the PI3K-Akt pathway. These pathways are integral to cell growth and survival influencing various physiological responses. The receptor shares functional relationships with proteins such as EDG1/S1P1 and EDG5/S1P2 which also bind S1P and are involved in similar signaling cascades. These interactions align EDG3/S1P3 with essential cellular processes emphasizing its role in maintaining homeostasis.

EDG3/S1P3 has associations with cardiovascular diseases and cancer. Its role in endothelial barrier function ties it to cardiovascular health where dysregulation can lead to conditions such as atherosclerosis. In cancer abnormal EDG3/S1P3 signaling can contribute to tumor growth and metastasis by influencing cell migration and survival. The receptor's involvement in these diseases links it to other proteins like VEGF which are essential in angiogenesis and vascular maintenance highlighting its therapeutic potential.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

See full target information S1PR3

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111290 PubMed39618500

2024

Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Daoquan Liu,Jianmin Liu,Yan Li,Lu Du,Qingqiong Cao,Liang Yang,Yongying Zhou,Ping Chen,Yuming Guo,Guang Zeng,Michael E DiSanto,Weidong Hu,Xinhua Zhang

International journal of molecular sciences 25: PubMed38928268

2024

Amelioration of Fibrosis via S1P Inhibition Is Regulated by Inactivation of TGF-β and SPL Pathways in the Human Cornea.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Nicholas,Sandip K Basu,Nawajes Mandal,Dimitrios Karamichos

Molecular and cellular biology 44:178-193 PubMed38767243

2024

TCF12 Transcriptionally Activates SPHK1 to Induce Osteosarcoma Angiogenesis by Promoting the S1P/S1PR4/STAT3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wo Li,Jitong Liu,Ting Cai,Xia Hu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23061 PubMed37389926

2023

Sphingosine 1-phosphate elicits a ROS-mediated proinflammatory response in human endometrial stromal cells via ERK5 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Isabelle Seidita,Ignazia Tusa,Matteo Prisinzano,Alessio Menconi,Francesca Cencetti,Silvia Vannuccini,Francesca Castiglione,Paola Bruni,Felice Petraglia,Caterina Bernacchioni,Elisabetta Rovida,Chiara Donati

Disease markers 2022:9119423 PubMed36438896

2022

Triptolide Inhibits the Biological Processes of HUVECs and HepG2 Cells via the Serine Palmitoyltransferase Long Chain Base Subunit 2/Sphingosine-1-Phosphate Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Jia,Shengnan Zhu,Mingfei Zhu,Lingyue Huang,Siyuan Xu,Yuqin Luo,Juan Xiao,Huazhen Su,Shaoyuan Huang,Qinyou Tan

International journal of molecular sciences 23: PubMed36361531

2022

Targeting SPHK1/PBX1 Axis Induced Cell Cycle Arrest in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhoujun Lin,Yin Li,Xiao Han,Zhenkun Fu,Zhenhuan Tian,Chenggang Li

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie

Biochimica et biophysica acta. Molecular and cell 1865:158632 PubMed31954174

2020

Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Del Gaudio,Ivana Sreckovic,Pablo Zardoya-Laguardia,Eva Bernhart,Christina Christoffersen,Saša Frank,Gunther Marsche,Sebastian E Illanes,Christian Wadsack

Clinical & translational oncology : official publi 21:1240-1249 PubMed30712233

2019

Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

A Palangi,N Shakhssalim,M Parvin,S Bayat,A Allameh

EBioMedicine 40:210-223 PubMed30587459

2018

S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Shen,Shujie Zhao,Shenyu Wang,Xiaohui Pan,Yunkun Zhang,Jingwen Xu,Yuqing Jiang,Haibo Li,Qiang Zhang,Jianbo Gao,Qin Yang,Yang Zhou,Shuheng Jiang,Huilin Yang,Zhigang Zhang,Rong Zhang,Jun Li,Dong Zhou

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EDG3/S1P3 抗体 [EPR4541(2)]

Anti-EDG3/S1P3 antibody [EPR4541(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

Western blot - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

OI-RD Scanning - Anti-EDG3/S1P3 antibody [EPR4541(2)] (AB126622)

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise