Anti-EDG2 /LPA-1 抗体 [EPR9710] (ab166903)
![Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-165262-anti-edg2-lpa-1-antibody-epr9710-western-blot.jpg "Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
- Suitable for: Indirect ELISA, WB, IHC-P, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-EDG2 /LPA-1 antibody [EPR9710]
EDG2 /LPA-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9710] to EDG2 /LPA-1 -
由来種
Rabbit -
アプリケーション
適用あり: Indirect ELISA, WB, IHC-P, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human, Recombinant fragment -
免疫原
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ポジティブ･コントロール
- Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9710 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166903の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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Indirect ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
ターゲット情報
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機能
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. -
組織特異性
Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles. - Information by UniProt
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参照データベース
- Entrez Gene: 1902 Human
- Omim: 602282 Human
- SwissProt: Q92633 Human
- Unigene: 126667 Human
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別名
- 5031439C20 antibody
- AI326300 antibody
- EDG 2 antibody
see all
画像
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Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution
Predicted band size: 39 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-165261-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-165260-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-165259-anti-edg2-lpa-1-antibody-epr9710-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Indirect ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-7-anti-edg2-lpa-1-antibody-epr9710-elisa-human-edg2lpa-1.jpg)
Indirect ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.co.jp/ps/products/166/ab166903/Images/ab166903-5-benefits-of-recombinant-antibodies.png)
Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab166903 は 11 報の論文で使用されています。
- Su Z et al. Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR-144-3p and upregulating LPCAT1 in oral squamous cell carcinoma. Laryngoscope Investig Otolaryngol 7:425-436 (2022). PubMed: 35434335
- Wu D et al. EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer. Cancer Cell Int 20:551 (2020). PubMed: 33292225
- Song K et al. Identification of genes with universally upregulated or downregulated expressions in colorectal cancer. J Gastroenterol Hepatol 34:880-889 (2019). PubMed: 30395690
- Akoume MY et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
- Cha YJ & Koo JS Expression of Autotaxin?Lysophosphatidate Signaling-Related Proteins in Breast Cancer with Adipose Stroma. Int J Mol Sci 20:N/A (2019). PubMed: 31035435