Anti-EDC4 抗体 (ab154026)
Key features and details
- Rabbit polyclonal to EDC4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EDC4 antibody
EDC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EDC4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment, corresponding to a region within amino acids 903-1181 of Human EDC4 (UniProt: Q6P2E9).
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ポジティブ･コントロール
- NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain whole cell lysates; HeLa cells; Human U87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154026の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 152 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 152 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). -
配列類似性
Belongs to the WD repeat EDC4 family.
Contains 4 WD repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > P-body. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23644 Human
- Entrez Gene: 234699 Mouse
- Entrez Gene: 361399 Rat
- Omim: 606030 Human
- SwissProt: Q6P2E9 Human
- SwissProt: Q3UJB9 Mouse
- SwissProt: Q3ZAV8 Rat
- Unigene: 75682 Human
see all -
別名
- autoantigen antibody
- Autoantigen Ge 1 antibody
- Autoantigen Ge-1 antibody
see all
画像
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Western blot - Anti-EDC4 antibody (ab154026)Anti-EDC4 antibody (ab154026) at 1/3000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 152 kDa
5% SDS PAGE -
Western blot - Anti-EDC4 antibody (ab154026)Anti-EDC4 antibody (ab154026) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 152 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EDC4 antibody (ab154026)Confocal Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EDC4 with ab154026 at 1/500 dilution (Green). Right image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDC4 antibody (ab154026)Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling EDC4 with ab154026 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154026 は論文での使用が確認できていません。