AB168851

Anti-EDC3 抗体

Name: Anti-EDC3 抗体 (ab168851) | Abcam
Brand: Abcam Limited
SKU: AB168851
Price: 76000 JPY
Availability: InStock

Anti-EDC3 antibody

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(4 Publications)

Rabbit Polyclonal EDC3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EDC3 aa 1-100.

別名を表示する

LSM16, YJDC, YJEFN2, PP844, EDC3, Enhancer of mRNA-decapping protein 3, LSM16 homolog, YjeF N-terminal domain-containing protein 2, YjeF domain-containing protein 1, YjeF_N2, hYjeF_N2

2 Images

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Immunoprecipitation - Anti-EDC3 antibody (AB168851)

Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-EDC3 antibody (ab168851)

Predicted band size: 56 kDa

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Unknown

Western blot - Anti-EDC3 antibody (AB168851)

All lanes:

Western blot - Anti-EDC3 antibody (ab168851) at 0.1 µg

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 56 kDa

true

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human EDC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96F86

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The EDC3 protein also known as Enhancer of mRNA Decapping 3 serves an essential role in mRNA processing and turnover. It functions mechanically within the RNA decay pathway aiding in the removal of the cap structure from mRNA a step important for mRNA degradation. EDC3 with a molecular mass of approximately 59 kDa is expressed in various tissues notably in brain and testis among others. It interacts with multiple RNA-binding proteins that support the assembly of mRNA decay machinery.

Biological function summary

EDC3 supports mRNA regulation by participating in mRNA decapping a regulatory process essential for controlling gene expression. EDC3 is a critical component of the decapping complex interacting with proteins like DCP1 and DCP2 facilitating efficient decapping of mRNA. The interaction of EDC3 with Pat1 another decapping activator enhances the mRNA decay process and ensures proper turnover of mRNA in the cell.

Pathways

EDC3 plays a part in the 5' to 3' mRNA decay pathway facilitating the removal of the cap structure leading to exonucleolytic degradation of mRNA. This pathway is important in mRNA degradation and turnover ensuring precise regulation of gene expression. EDC3 is related to proteins such as LSM1 and XRN1 which cooperate within the 5' to 3' exonucleolytic decay pathway promoting efficient mRNA degradation.

EDC3 alterations may associate with neurological conditions and cancer. Studies suggest that disruptions in mRNA decay where EDC3 plays a central role can lead to improper gene expression contributing to neuronal dysfunctions in neurological diseases. Also anomalies in decay pathways may link to oncogenic processes potentially pointing to EDC3's involvement in cancer development. EDC3’s interaction with proteins like DCP2 and XRN1 in the context of these disorders reveals the significance of RNA processing in maintaining cellular homeostasis and preventing disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.

See full target information EDC3

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 185:2035-2056.e33 PubMed35688132

2022

The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Erinc Hallacli,Can Kayatekin,Sumaiya Nazeen,Xiou H Wang,Zoe Sheinkopf,Shubhangi Sathyakumar,Souvarish Sarkar,Xin Jiang,Xianjun Dong,Roberto Di Maio,Wen Wang,Matthew T Keeney,Daniel Felsky,Jackson Sandoe,Aazam Vahdatshoar,Namrata D Udeshi,D R Mani,Steven A Carr,Susan Lindquist,Philip L De Jager,David P Bartel,Chad L Myers,J Timothy Greenamyre,Mel B Feany,Shamil R Sunyaev,Chee Yeun Chung,Vikram Khurana

Cell chemical biology 28:463-474.e7 PubMed33357462

2020

Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Jeremy A Schofield,Zhenkun Na,Tanja Hann,Matthew D Simon,Sarah A Slavoff

Molecular cell 68:144-157.e5 PubMed28965817

2017

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.

Applications

Unspecified application

Species

Unspecified reactive species

Arnaud Hubstenberger,Maïté Courel,Marianne Bénard,Sylvie Souquere,Michèle Ernoult-Lange,Racha Chouaib,Zhou Yi,Jean-Baptiste Morlot,Annie Munier,Magali Fradet,Maëlle Daunesse,Edouard Bertrand,Gérard Pierron,Julien Mozziconacci,Michel Kress,Dominique Weil

Nature chemical biology 13:174-180 PubMed27918561

2016

A human microprotein that interacts with the mRNA decapping complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia G D'Lima,Jiao Ma,Lauren Winkler,Qian Chu,Ken H Loh,Elizabeth O Corpuz,Bogdan A Budnik,Jens Lykke-Andersen,Alan Saghatelian,Sarah A Slavoff

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EDC3 抗体

Anti-EDC3 antibody

Immunoprecipitation - Anti-EDC3 antibody (AB168851)

Western blot - Anti-EDC3 antibody (AB168851)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise