Anti-ECM29 抗体 (ab28666)
Key features and details
- Rabbit polyclonal to ECM29
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ECM29 antibody -
製品の詳細
Rabbit polyclonal to ECM29 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ECM29 aa 1821-1839 (C terminal).
Sequence:RPELQEKAALLKKTLENLE
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ポジティブ・コントロール
- WB: HeLa cell lysate. ICC/IF: HeLa cells, K-562 and NTERA-2 cl.D1 lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28666の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/200. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
ECM29 appears to be one of three proteins which associate significantly with proteasomes. ECM29 has been shown to enhance the stability of the proteasome. ECM29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that ECM29 tethers the regulatory complex to the proteasome. -
細胞内局在
Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm › cytoskeleton › centrosome. Nucleus. -
参照データベース
- Entrez Gene: 23392 Human
- SwissProt: Q5VYK3 Human
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別名
- ECM29 homolog, proteasome accessory protein antibody
- FLJ22036 antibody
- KIAA0368 antibody
see all
画像
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All lanes : Anti-ECM29 antibody (ab28666) at 1/250 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K-562 whole cell lysate
Lane 3 : NTERA-2 cl.D1 whole cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody at 1/2500 dilution
Predicted band size: 205 kDa
Observed band size: 204 kDa why is the actual band size different from the predicted?Additional bands of 70 and 160 kDa observed might correspond to interacting partners of dynamin intermediate chain and kinesin intermediate chain respectively.
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Immunofluorescence analysis of HeLa cells labelling ECM29 with ab28666 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at room temperature was used as the secondary antibody (green) (a). F-actin (was stained with Rhodamine Phalloidin (red) (c) and Nuclear DNA was labelled with DAPI (blue) (b). (d) represents the merged image showing cytoplasmic localization. (e) shows the no primary antibody control. The images were captured at 60X magnification.
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Anti-ECM29 antibody (ab28666) (dilution of 1/200) + HeLa cell extract
Predicted band size: 205 kDa
Observed band size: 205 kDa
Detection of ECM29 in HeLa cells. -
Immunofluorescent staining of Ecm29 in HeLa cells using ab28666 (1/200 dilution).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab28666 は 4 報の論文で使用されています。
- Ibañez-Vega J et al. Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse. Front Cell Dev Biol 9:650817 (2021). PubMed: 34055780
- Lee M et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219:N/A (2020). PubMed: 31910261
- Zhang J et al. Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency. Exp Ther Med 15:3189-3196 (2018). PubMed: 29545834
- Miettinen TP et al. Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib. EMBO J 37:N/A (2018). PubMed: 29669860