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リコンビナントRabMAb

Anti-ECHS1 抗体 [EPR11784(B)] (ab170108)

Submit a review Submit a question References (3)
Flow Cytometry (Intracellular) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
  • Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11784(B)] to ECHS1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ECHS1 antibody [EPR11784(B)]
    ECHS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11784(B)] to ECHS1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human ECHS1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30084

  • ポジティブ･コントロール

    • Fetal liver and HeLa lysates; Human cardiac muscle and liver tissues; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170108の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 関連性

    ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.

  • 細胞内局在

    Mitochondrion matrix.

  • 参照データベース

    • 別名

      • C80529 antibody
      • EC 4.2.1.17 antibody
      • ECHS 1 antibody
      • Enoyl CoA hydratase 1 antibody
      • Enoyl CoA hydratase mitochondrial antibody
      • Enoyl CoA hydratase short chain 1 mitochondrial antibody
      • Enoyl Coenzyme A hydratase short chain 1 antibody
      • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
      • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
      • OTTHUMP00000020811 antibody
      • SCEH antibody
      • Short chain enoyl CoA hydratase antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Flow Cytometry (Intracellular) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ECHS1with ab170108at 1/100 dilution (red) compared toa rabbit IgG negative control (green). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ECHS1 with ab170108 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Western blot - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      All lanes : Anti-ECHS1 antibody [EPR11784(B)] (ab170108) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
      Anti-ECHS1 antibody [EPR11784(B)] (ab170108)

    参考文献 (3)

    ab170108 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab170108 は 3 報の論文で使用されています。

    • Yang Y  et al. Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. Virol J 18:39 (2021). PubMed: 33602268
    • Luo Z  et al. Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity. J Cell Mol Med 25:8863-8876 (2021). PubMed: 34402182
    • Bhopale KK  et al. Proteomic Profiling of Liver and Plasma in Chronic Ethanol Feeding Model of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice. Alcohol Clin Exp Res 41:1675-1685 (2017). PubMed: 28792616

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