Anti-ECHS1 抗体 [EPR11784(B)] (ab170108)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11784(B)] to ECHS1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ECHS1 antibody [EPR11784(B)]
ECHS1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11784(B)] to ECHS1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human ECHS1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: P30084 -
ポジティブ・コントロール
- Fetal liver and HeLa lysates; Human cardiac muscle and liver tissues; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11784(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway. -
細胞内局在
Mitochondrion matrix. -
参照データベース
- Entrez Gene: 1892 Human
- Entrez Gene: 93747 Mouse
- Entrez Gene: 140547 Rat
- Omim: 602292 Human
- SwissProt: P30084 Human
- SwissProt: Q8BH95 Mouse
- SwissProt: P14604 Rat
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別名
- C80529 antibody
- EC 4.2.1.17 antibody
- ECHS 1 antibody
see all
画像
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ECHS1with ab170108at 1/100 dilution (red) compared toa rabbit IgG negative control (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ECHS1 with ab170108 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECHS1 antibody [EPR11784(B)] (ab170108)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ECHS1 with ab170108 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ECHS1 antibody [EPR11784(B)] (ab170108) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab170108 は 3 報の論文で使用されています。
- Yang Y et al. Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. Virol J 18:39 (2021). PubMed: 33602268
- Luo Z et al. Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity. J Cell Mol Med 25:8863-8876 (2021). PubMed: 34402182
- Bhopale KK et al. Proteomic Profiling of Liver and Plasma in Chronic Ethanol Feeding Model of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice. Alcohol Clin Exp Res 41:1675-1685 (2017). PubMed: 28792616