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Anti-EBV Latent Membrane Protein 1 抗体 [CS 1-4] (ab78113)

Reviews (4)Q&A (3)References (45)

Key features and details

  • Mouse monoclonal [CS 1-4] to EBV Latent Membrane Protein 1
  • Suitable for: WB, IP, IHC-P, IHC-Fr
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]
    EBV Latent Membrane Protein 1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CS 1-4] to EBV Latent Membrane Protein 1

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for Epstein-Barr virus infected cells expressing the latent membrane protein (LMP). This is in fact four pooled antibodies which collectively detect LMP's encoded by each of 20 geographically distinct EBV isolates.

  • アプリケーション

    適用あり: WB, IP, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Epstein-Barr virus

  • 免疫原

    Recombinant fragment corresponding to EBV Latent Membrane Protein 1.
    Database link: P03230

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Affinity chromatography

  • ポリ/モノ

    モノクローナル

  • クローン名

    CS 1-4

  • ミエローマ

    x63-Ag8.653

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab78113の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.

  • 参照データベース

    • 別名

      • BNLF1 antibody
      • EBV antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      • Latent membrane protein 1 antibody
      • LMP1 antibody
      see all

    参考文献 (45)

    ab78113 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab78113 は 45 報の論文で使用されています。

    • Liu W  et al. Epstein-Barr Virus Regulates Endothelin-1 Expression through the ERK/FOXO1 Pathway in EBV-Associated Gastric Cancer. Microbiol Spectr 11:e0089822 (2023). PubMed: 36475746
    • Xu M  et al. Establishment and characterization of two Epstein-Barr virus-positive gastric cancer cell lines with epitheliotropic M81 strain undergoing distinct viral and altered cellular expression profiles. J Med Virol 95:e28387 (2023). PubMed: 36478267
    • Lange PT & Damania B Epstein-Barr Virus-Positive Lymphomas Exploit Ectonucleotidase Activity To Limit Immune Responses and Prevent Cell Death. mBio 14:e0345922 (2023). PubMed: 36786572
    • Saikumar Lakshmi P  et al. Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapies. Life Sci Alliance 6:N/A (2023). PubMed: 36878637
    • Deng Y  et al. STIM1-regulated exosomal EBV-LMP1 empowers endothelial cells with an aggressive phenotype by activating the Akt/ERK pathway in nasopharyngeal carcinoma. Cell Oncol (Dordr) 46:987-1000 (2023). PubMed: 36917356
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EBV transformed B cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Specification
    EBV transformed B cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 08 2015

    Immunocytochemistry abreview for Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Sample
    Mouse Cultured Cells (lymphocyte)
    Specification
    lymphocyte
    Permeabilization
    Yes - acetone
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Western blot abreview for Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% gel)
    Sample
    Human Cell lysate - whole cell (PBMC)
    Specification
    PBMC
    Treatment
    EBV for 14days
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Immunocytochemistry abreview for Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Sample
    Mouse Cultured Cells (lymphoma)
    Specification
    lymphoma
    Permeabilization
    Yes - Acetone
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 23 2015

    Question

    Vielen, lieben Dank für Ihre detaillierte Antwort!

    Ich bräuchte allerdings leider wirklich einen Antikörper, der extracellulär bindet.

    Plant abcam zufällig, so einen herzustellen, oder hat vielleicht schon einen, der nur noch nicht auf dem Markt ist?

    Vielen, herzlichen DAnk!
    Frohe Weihnachten!

    Read More
    Answer

    Vielen Dank für Ihre Antwort.

    Leider muss ich Ihnen bestätigen, dass wir keinen Antikörper gegen die extrazelluläre Domäne in Produktion haben.

    Ich werde diesen Vorschlag allerdings gerne weitergeben. Leider kann ich damit aber nicht garantieren, dass dieser Antikörper tatsächlich ins Programm aufgenommen wird.

    Ich möchte Sie deshalb gerne darauf hinweisen, dass wir einen Service für Spezialanfertigungen von Antikörpern haben.

    http://www.epitomics.com/services/

    Bitte lassen Sie mich wissen, ob Sie interessiert sind. Ich werde Sie dann gerne an die zuständige Person weiterleiten.

    Ich wünsche Ihnen ein Frohes Neues Jahr 2013 und verbleibe

    Read More

    Question

    Would ab78113 recognise Rhesus monkey tissue?

    Read More
    Answer

    Thank you for contacting Abcam. I have tried blasting the human EBV-LMP1 sequences against the rhesus monkey database and I cannot seem to get a hit for the rhesus EBV sequence (the only hit is for unnamed protein product, #21103, which has a 70% homolgy for huEBV). I have also tried to find the EBV rhesus sequence in swiss prot, but I cannot find it there either. Therefore I cannot confirm if ab78113 would react with the rhesus monkey tissue. I was wondering if you knew of the accession number for the sequence (rhesus)? If so, I could do the alignment for see if the antibody would work for you. I look forward to your reply.

    Read More

    Question

    Dear sir\madam, thanks for your cooperation and response. please we also looking for a secondary antibody: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) and Anti-beta Actin antibody [AC-15] (ab6276). also please let me know if it is compatible with the three previous primary antibodies.

    Read More
    Answer

    Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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