Anti-EBP50/NHERF-1 抗体 (ab3452)
Key features and details
- Rabbit polyclonal to EBP50/NHERF-1
- Suitable for: WB, IP, IHC-Fr, ICC, IHC-P
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-EBP50/NHERF-1 antibody
EBP50/NHERF-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EBP50/NHERF-1 -
由来種
Rabbit -
特異性
Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein. -
アプリケーション
適用あり: WB, IP, IHC-Fr, ICC, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Dog, Human
交差が予測される動物種: Rabbit, Cow, Cynomolgus monkey
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免疫原
Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
Sequence:RSASSDTSEELNSQDSP
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ポジティブ･コントロール
- WB: MCF7, T47D, SK-BR-3, HeLa, HepG2; Caco-2. IHC: mouse airway ICC: T47D cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| IP |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 15703401
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| ICC |
Use a concentration of 2 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 15703401 |
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ICC
Use a concentration of 2 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC. -
組織特異性
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. -
関連疾患
Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization). -
配列類似性
Contains 2 PDZ (DHR) domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells. - Information by UniProt
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参照データベース
- Entrez Gene: 505242 Cow
- Entrez Gene: 9368 Human
- Entrez Gene: 26941 Mouse
- Entrez Gene: 100009196 Rabbit
- Entrez Gene: 59114 Rat
- Omim: 604990 Human
- SwissProt: Q3SZK8 Cow
- SwissProt: O14745 Human
see all -
別名
- EBP 50 antibody
- EBP50 antibody
- Ezrin radixin moesin binding phosphoprotein 50 antibody
see all
画像
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Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (ab3452)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized T-47D cells staining EBP50/NHERF-1 with ab3452 at 2µg/ml, and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control used no primary antibody.
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 0.2 µg/ml
Lane 1 : Membrane enriched extracts of MCF7 (human breast adenocarcinoma cell line)
Lane 2 : Membrane enriched extracts of T-47D (Human ductal breast epithelial tumor cell line)
Lane 3 : Membrane enriched extracts of SK-BR-3 (Human mammary gland adenocarcinoma cell line)
Lane 4 : Membrane enriched extracts of MDA-MB-231 (human breast adenocarcinoma cell line)
Lane 5 : Membrane enriched extracts of HeLa (human epithelial cell line from cervix adenocarcinoma)
Lane 6 : Membrane enriched extracts of HepG2 (human liver hepatocellular carcinoma cell line)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa -
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1 µg/ml
Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 50 kDa -
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole cell lysate prepared from human HepG2 cells
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 40 seconds
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole tissue lysate prepared from mouse liver
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 30 seconds
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (42)
ab3452 は 42 報の論文で使用されています。
- Friedman PA et al. RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein. J Biol Chem 298:101836 (2022). PubMed: 35307350
- Kammala AK et al. Na+/H+ Exchanger Regulatory Factor 1 Mediates the Pathogenesis of Airway Inflammation in a Murine Model of House Dust Mite-Induced Asthma. J Immunol 206:2301-2311 (2021). PubMed: 33952618
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Capdevielle C et al. HDAC inhibition induces expression of scaffolding proteins critical for tumor progression in pediatric glioma: focus on EBP50 and IRSp53. Neuro Oncol 22:550-562 (2020). PubMed: 31711240
- Kammala AK et al. Sodium Hydrogen Exchanger Regulatory Factor-1 (NHERF1) Regulates Fetal Membrane Inflammation. Int J Mol Sci 21:N/A (2020). PubMed: 33092043