Anti-EBP1 抗体 [EPR14568(B)] (ab180603)
![Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14568(B)] (ab180603)](https://www.abcam.co.jp/ps/products/180/ab180603/Images/ab180603-211977-anti-ebp1-antibody-epr14568b-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14568(B)] (ab180603)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14568(B)] to EBP1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EBP1 antibody [EPR14568(B)]
EBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14568(B)] to EBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, 293T, Jurkat and A431 lysates; Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR14568(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180603の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
|
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| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). |
|
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
組織特異性
Expressed in several cell lines tested, including primary and transformed cell lines. -
配列類似性
Belongs to the peptidase M24 family. -
翻訳後修飾
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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参照データベース
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
- Unigene: 573018 Human
see all -
別名
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-EBP1 antibody [EPR14568(B)] (ab180603)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat cells labeling EBP1 with ab180603 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green),followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
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![Western blot - Anti-EBP1 antibody [EPR14568(B)] (ab180603)](https://www.abcam.co.jp/ps/products/180/ab180603/Images/ab180603-211976-anti-ebp1-antibody-epr14568b-western-blot.jpg)
Western blot - Anti-EBP1 antibody [EPR14568(B)] (ab180603)All lanes : Anti-EBP1 antibody [EPR14568(B)] (ab180603) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
![Anti-EBP1 antibody [EPR14568(B)] (ab180603)](https://www.abcam.co.jp/ps/products/180/ab180603/Images/ab180603-3-benefits-of-recombinant-antibodies.png)
Anti-EBP1 antibody [EPR14568(B)] (ab180603)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab180603 は論文での使用が確認できていません。