Anti-EBP1 抗体 (ab186846)
Key features and details
- Rabbit polyclonal to EBP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EBP1 antibody
EBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EBP1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human EBP1. The exact sequence is proprietary.
Database link: Q9UQ80 -
ポジティブ･コントロール
- Extracts of mouse liver, BXPC3, Hela, PC12, NIH 3T3, K562, 22RV1 and HT29 cell lines.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 44 kDa.
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| IHC-P |
1/50 - 1/200.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 44 kDa. |
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IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
組織特異性
Expressed in several cell lines tested, including primary and transformed cell lines. -
配列類似性
Belongs to the peptidase M24 family. -
翻訳後修飾
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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参照データベース
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
- Unigene: 573018 Human
see all -
別名
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
画像
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Western blot - Anti-EBP1 antibody (ab186846)All lanes : Anti-EBP1 antibody (ab186846) at 1/500 dilution
Lane 1 : Extract of mouse liver cell line
Lane 2 : Extract of BXPC3 cell line
Lane 3 : Extract of Hela cell line
Lane 4 : Extract of PC12 cell line
Lane 5 : Extract of NIH 3T3 cell line
Lane 6 : Extract of K562 cell line
Lane 7 : Extract of 22RV1 cell line
Lane 8 : Extract of HT29 cell line
Predicted band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody (ab186846)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling EBP1 with ab186846 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab186846)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186846. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186846 は 1 報の論文で使用されています。
- Hwang I & Ahn JY Dysregulation of Epigenetic Control Contributes to Schizophrenia-Like Behavior in Ebp1+/- Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32283721