AB186846

Anti-EBP1 抗体

Name: Anti-EBP1 抗体 (ab186846) | Abcam
Brand: Abcam Limited
SKU: AB186846
Price: 77000 JPY
Availability: InStock

Anti-EBP1 antibody

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(3 Publications)

Rabbit Polyclonal EBP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PA2G4.

別名を表示する

EBP1, PA2G4, Proliferation-associated protein 2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1, hG4-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (AB186846)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (AB186846)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186846. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody (AB186846)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody (AB186846)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling EBP1 with ab186846 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-EBP1 antibody (AB186846)

All lanes:

Western blot - Anti-EBP1 antibody (ab186846) at 1/500 dilution

Lane 1:

Extract of mouse liver cell line

Lane 2:

Extract of BXPC3 cell line

Lane 3:

Extract of Hela cell line

Lane 4:

Extract of PC12 cell line

Lane 5:

Extract of NIH 3T3 cell line

Lane 6:

Extract of K562 cell line

Lane 7:

Extract of 22RV1 cell line

Lane 8:

Extract of HT29 cell line

Predicted band size: 44 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PA2G4. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ80

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The EBP1 protein also known as ErbB3-binding protein 1 plays an important role in cellular mechanisms. With a mass of about 39 kDa EBP1 is widely expressed in various tissues including the heart liver and brain. The protein acts as an RNA-binding protein and it has demonstrated influence in the regulation of ribosomal RNA synthesis and processing. EBP1 also interacts with multiple other proteins which aid in its regulatory roles in the cells.

Biological function summary

EBP1 functions as a part of the cellular machinery involved in growth and differentiation. It associates with the eukaryotic translation initiation factor 4G (eIF4G) as part of a complex which modulates the translation of certain messenger RNAs. Furthermore EBP1 can function as a transcriptional co-repressor in the nucleus where it influences gene expression by interacting with DNA. Its activity in these roles highlights its important function in cell cycle regulation and cellular proliferation.

Pathways

Many signaling mechanisms engage EBP1. The PI3K/AKT pathway sees participation from EBP1 where it downstream affects cell survival and growth. EBP1 also interacts with the NF-κB pathway contributing to the regulation of immune and stress responses. In these pathways interactions with key proteins like AKT and NF-κB p65 emphasize its role in integrating signaling cues for responses to environmental stimuli.

EBP1 has shown associations with cancer and neurodegenerative diseases. Its connection with cancer is evident as EBP1 influences cell proliferation and can affect tumor suppressor pathways sometimes interacting with proteins like HER2. In neurodegenerative diseases deregulation of EBP1 function has been seen in conditions such as Alzheimer's potentially interacting with proteins involved in tau phosphorylation. Both examples demonstrate the biological significance of EBP1 in maintaining normal physiological function and its contributions to pathology when dysregulated.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

See full target information PA2G4

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:365-379 PubMed37712850

2023

PA2G4/EBP1 ubiquitination by PRKN/PARKIN promotes mitophagy protecting neuron death in cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Inwoo Hwang,Byeong-Seong Kim,Ho Yun Lee,Sung-Woo Cho,Seung Eun Lee,Jee-Yin Ahn

BioFactors (Oxford, England) 48:683-698 PubMed35080047

2022

Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tan,Huizhuo Xu,Bolin Chen,Tianqi Duan,Kangcheng Liu,Jing Zou

International journal of molecular sciences 21: PubMed32283721

2020

Dysregulation of Epigenetic Control Contributes to Schizophrenia-Like Behavior in Ebp1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Inwoo Hwang,Jee-Yin Ahn

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EBP1 抗体

Anti-EBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (AB186846)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP1 antibody (AB186846)

Western blot - Anti-EBP1 antibody (AB186846)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise