Anti-EB3 (phospho S162) 抗体 [KT113] (ab179877)
![Western blot - Anti-EB3 (phospho S162) antibody [KT113] (ab179877)](https://www.abcam.co.jp/ps/products/179/ab179877/Images/ab179877-209285-anti-eb3-antibody-kt113-western-blot.jpg "Western blot - Anti-EB3 (phospho S162) antibody [KT113] (ab179877)")
Key features and details
- Mouse monoclonal [KT113] to EB3 (phospho S162)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-EB3 (phospho S162) antibody [KT113]
EB3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [KT113] to EB3 (phospho S162) -
由来種
Mouse -
特異性
ab179877 does not cross-react with EB3 if not phosphorylated at S162. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human EB3 (phospho S162). Conjugated to carrier protein.
Database link: Q9UPY8 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
KT113 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/20000. Predicted molecular weight: 31 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/20000. Predicted molecular weight: 31 kDa. |
ターゲット情報
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機能
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
組織特異性
Predominantly expressed in brain and muscle. -
配列類似性
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
ドメイン
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
細胞内局在
Cytoplasm > cytoskeleton. Associated with the microtubule network. - Information by UniProt
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参照データベース
- Entrez Gene: 22924 Human
- Omim: 605788 Human
- SwissProt: Q9UPY8 Human
- Unigene: 515860 Human
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別名
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
see all
画像
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Western blot - Anti-EB3 (phospho S162) antibody [KT113] (ab179877)All lanes : Anti-EB3 (phospho S162) antibody [KT113] (ab179877) at 1/5000 dilution
Lane 1 : HEK cells expressing wild type EB3 (S162 phosphorylated) fused with GFP
Lane 2 : HEK cells expressing non-phosphorylated EB3 fused with GFP
Predicted band size: 31 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab179877 は論文での使用が確認できていません。