Anti-EB2 抗体 [KT52] (ab45767)
![Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (ab45767)](https://www.abcam.co.jp/ps/products/45/ab45767/Images/ab45767-57852-anti-eb2-antibody-k52-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (ab45767)")
Key features and details
- Rat monoclonal [KT52] to EB2
- Suitable for: ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG2b
製品の概要
-
製品名
Anti-EB2 antibody [KT52]
EB2 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [KT52] to EB2 -
由来種
Rat -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length EB2 protein (Human)
-
ポジティブ･コントロール
- CHO cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
KT52 -
アイソタイプ
IgG2b -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45767の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
組織特異性
Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes. -
配列類似性
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
ドメイン
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
細胞内局在
Cytoplasm > cytoskeleton. Associated with the microtubule network. Accumulates at the plus end of microtubules. - Information by UniProt
-
別名
- APC binding protein EB1 antibody
- APC binding protein EB2 antibody
- APC-binding protein EB2 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-EB2 antibody [KT52] (ab45767)Immunofluorescence staining of EB2 in CHO cells transfected with EB2 gene expression constructs.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab45767 は 7 報の論文で使用されています。
- Ong HW et al. Discovery of a Potent and Selective CDKL5/GSK3 Chemical Probe That Is Neuroprotective. ACS Chem Neurosci 14:1672-1685 (2023). PubMed: 37084253
- Van Bergen NJ et al. Abnormalities of mitochondrial dynamics and bioenergetics in neuronal cells from CDKL5 deficiency disorder. Neurobiol Dis N/A:105370 (2021). PubMed: 33905871
- Terzic B et al. Temporal manipulation of Cdkl5 reveals essential postdevelopmental functions and reversible CDKL5 deficiency disorder-related deficits. J Clin Invest 131:N/A (2021). PubMed: 34651584
- Gong L et al. WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. Front Oncol 10:461 (2020). PubMed: 32426268
- Tang S et al. Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nat Commun 10:2655 (2019). PubMed: 31201320
- Baltussen LL et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J 37:N/A (2018). PubMed: 30266824
- Asanov A et al. A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum-plasma membrane junctions. Cell Calcium 54:246-56 (2013). PubMed: 23871111