Name: Anti-EAAT1 antibody [EPR12686]
Brand: RabMAb
SKU: ab181036
Rating: 5 (3 reviews)

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Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12686] to EAAT1
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品名

Anti-EAAT1 antibody [EPR12686]
EAAT1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR12686] to EAAT1
由来種

Rabbit
特異性

Unsuitable for human ICC/IF.
アプリケーション

適用あり: IHC-P, WB, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
交差が予測される動物種: Monkey
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Mouse brain, rat brain and human cerebellum lysates. IHC-P: Human , Mouse and Rat cerebral cortex tissue sections. ICC/IF: Mouse and rat primary neural / glia cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR12686
アイソタイプ

IgG
研究分野
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
- Anti-EAAT1 antibody [EPR12686] - BSA and Azide free (ab240235)
- Alexa Fluor® 568 Anti-EAAT1 antibody [EPR12686] (ab313041)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab181036の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF		1/50.

特記事項
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF 1/50.

機能

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
組織特異性

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
関連疾患

Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
配列類似性

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
翻訳後修飾

Glycosylated.
細胞内局在

Membrane.
Target information above from: UniProt accession P43003 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 204834 Mouse
- Unigene: 34134 Rat
see all
別名
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
- Excitatory amino acid transporter 1 antibody
- FLJ25094 antibody
- GLAST antibody
- GLAST-1 antibody
- GLAST1 antibody
- Glial high affinity glutamate transporter antibody
- glutamate/aspartate transporter, high affinity, sodium-dependent antibody
- High affinity neuronal glutamate transporter antibody
- Slc1a3 antibody
- Sodium dependent glutamate/aspartate transporter antibody
- Sodium-dependent glutamate/aspartate transporter 1 antibody
- Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
- Solute carrier family 1 member 3 antibody
see all

Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 59 kDa

Exposure time: 10 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

We recommend not to boil the samples after lysis to get desired WB results.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

Lane 1 : Mouse brain
Lane 2 : Mouse hippocampus
Lane 3 : Rat hippocampus
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural / glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution + Human cerebellum at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST

The band above (100KDa) is dimer
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab181036 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181036 は 7 報の論文で使用されています。

Zheng J et al. GLP-1 improves the supportive ability of astrocytes to neurons by promoting aerobic glycolysis in Alzheimer's disease. Mol Metab 47:101180 (2021). PubMed: 33556642
Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
Sun N et al. DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients. Pediatr Investig 5:106-111 (2021). PubMed: 34179706
Sipe GO et al. Astrocyte glutamate uptake coordinates experience-dependent, eye-specific refinement in developing visual cortex. Glia 69:1723-1735 (2021). PubMed: 33675674
Sakami S et al. Müller glia phagocytose dead photoreceptor cells in a mouse model of retinal degenerative disease. FASEB J 33:3680-3692 (2019). PubMed: 30462532
Wang R et al. Astrocytic JWA deletion exacerbates dopaminergic neurodegeneration by decreasing glutamate transporters in mice. Cell Death Dis 9:352 (2018). PubMed: 29500411
Liu J et al. Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity. Glia 66:62-77 (2018). IHC-P ; Human . PubMed: 28925561

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Bos taurus Cell lysate - whole cell (Lane 1=no sample (negative control) Lane 2=bovine)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

Lane 1=no sample (negative control) Lane 2=bovine

Blocking step

No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 28°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alexis Hruby

Verified customer

投稿 Jul 18 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Common marmoset Tissue sections (Cochlea)

Permeabilization

Specification

Cochlea

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 27 2022

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Citric acid 0.05% Tween20

Sample

Monkey Tissue sections (brain)

Specification

brain

Permeabilization

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 27 2014

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (7)

レビューと Q&A

Western blot abreview for Anti-EAAT1 antibody [EPR12686]

Immunohistochemistry (Frozen sections) abreview for Anti-EAAT1 antibody [EPR12686]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EAAT1 [EPR12686] antibody