Anti-E2F6 抗体 [TFE61] (ab11952)
![Western blot - Anti-E2F6 antibody [TFE61] (ab11952)](https://www.abcam.co.jp/ps/products/11/ab11952/Images/ACFAF8.jpg "Western blot - Anti-E2F6 antibody [TFE61] (ab11952)")
Key features and details
- Mouse monoclonal [TFE61] to E2F6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-E2F6 antibody [TFE61]
E2F6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TFE61] to E2F6 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human)
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ポジティブ･コントロール
- Hela cells or K-562 nuclear extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
TFE61 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11952の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
組織特異性
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
配列類似性
Belongs to the E2F/DP family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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別名
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
画像
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Western blot - Anti-E2F6 antibody [TFE61] (ab11952)
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab11952 は 6 報の論文で使用されています。
- Junghof J et al. CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells. NPJ Regen Med 7:14 (2022). PubMed: 35110584
- Han N et al. LncRNA MATN1-AS1 prevents glioblastoma cell from proliferation and invasion via RELA regulation and MAPK signaling pathway. Ann Transl Med 7:784 (2019). PubMed: 32042800
- Pei Y et al. EBV Nuclear Antigen 3C Mediates Regulation of E2F6 to Inhibit E2F1 Transcription and Promote Cell Proliferation. PLoS Pathog 12:e1005844 (2016). PubMed: 27548379
- Hansen HG et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). PubMed: 26657798
- Furuta M et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . PubMed: 19843643
- Kozaki K et al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB ; Human . PubMed: 18381414