Name: Anti-E2F4 antibody [EPR8259]
Brand: RabMAb
SKU: ab150360
Rating: 3 (3 reviews)

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Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8259] to E2F4
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Carrier Free

製品名

Anti-E2F4 antibody [EPR8259]
E2F4 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR8259] to E2F4
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP
種交差性

交差種: Human
免疫原

Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
Database link: Q16254
ポジティブ･コントロール
- WB: HEK293T, K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR8259
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-E2F4 antibody [EPR8259] - BSA and Azide free (ab240118)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human E2F4 knockout HEK-293T cell line (ab266119)
KO cell lysates
- Human E2F4 knockout HEK-293T cell lysate (ab257932)
Positive Controls
Recombinant Protein
- Recombinant Human E2F4 protein (ab152352)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab150360の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(2)	1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt,ICC/IF or IP.

機能

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
組織特異性

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
配列類似性

Belongs to the E2F/DP family.
発生段階

Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
翻訳後修飾

Differentially phosphorylated in vivo.
細胞内局在

Nucleus.
Target information above from: UniProt accession Q16254 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1874 Human
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
別名
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
- E2F-4 antibody
- E2F4 antibody
- E2F4_HUMAN antibody
- p107/p130 binding protein antibody
- Transcription factor E2F 4 antibody
- Transcription factor E2F4 antibody
see all

Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)

All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : E2F4 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)

All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-E2F4 antibody [EPR8259] (ab150360)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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ab150360 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab150360 は 6 報の論文で使用されています。

Zheng Q et al. Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. J Cancer 12:1792-1803 (2021). PubMed: 33613768
Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
Wang SA et al. USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy. Sci Rep 10:20870 (2020). PubMed: 33257797
Dhanasekaran K et al. Unraveling the role of aurora A beyond centrosomes and spindle assembly: implications in muscle differentiation. FASEB J 33:219-230 (2019). PubMed: 29995440
Li LL et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). PubMed: 24886710

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Pancreas)

Gel Running Conditions

Reduced Denaturing (4-12)

Loading amount

40 µg

Specification

Pancreas

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 30 2022

Application

Flow Cytometry

Sample

Human Cell (TIG3 cells)

Permeabilization

Yes - 0.1% TritonX-100

Gating Strategy

G2/M cells stained with DAPI

Specification

TIG3 cells

Fixation

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 25 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (eHAP1 cells)

Gel Running Conditions

Reduced Denaturing (8)

Loading amount

20 µg

Specification

eHAP1 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 10 2018

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

発生段階

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (6)

レビューと Q&A

Western blot abreview for Anti-E2F4 antibody [EPR8259]

Flow Cytometry abreview for Anti-E2F4 antibody [EPR8259]

Western blot abreview for Anti-E2F4 antibody [EPR8259]