Anti-E2F1 抗体 (ab94888)
Key features and details
- Rabbit polyclonal to E2F1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-E2F1 antibody
E2F1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to E2F1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to amino acids 183-433 of Human E2F1 (NM_005225).
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ポジティブ･コントロール
- K562 cell lysate and Human fetal tonsil tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94888の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/50 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/50 - 1/200. |
ターゲット情報
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機能
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
配列類似性
Belongs to the E2F/DP family. -
翻訳後修飾
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Entrez Gene: 399489 Rat
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- SwissProt: O09139 Rat
- Unigene: 654393 Human
see all -
別名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
画像
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Western blot - Anti-E2F1 antibody (ab94888)Anti-E2F1 antibody (ab94888) at 1/500 dilution + K562 cell lysate
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody (ab94888)ab94888, at a 1/100 dilution, staining E2F1 in formalin fixed, paraffin embedded Human fetal tonsil tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab94888 は 4 報の論文で使用されています。
- Chen X et al. GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity. J Cell Physiol 236:4050-4065 (2021). PubMed: 33174204
- Zhou L et al. Wnt5a Promotes Cortical Neuron Survival by Inhibiting Cell-Cycle Activation. Front Cell Neurosci 11:281 (2017). PubMed: 29033786
- Prystowsky M et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). PubMed: 24026482
- Kim SY & Rane SG The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors. Development 138:1903-12 (2011). PubMed: 21490060