Anti-E1B-AP5 抗体 [EP2633Y] (ab68480)
![Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.co.jp/ps/products/68/ab68480/Images/ab68480-397737-anti-e1b-ap5-antibody-ep2633y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)")
Key features and details
- Rabbit monoclonal [EP2633Y] to E1B-AP5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-E1B-AP5 antibody [EP2633Y]
E1B-AP5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2633Y] to E1B-AP5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human E1B-AP5 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: Wild-type HEK293T, Raji, and HeLa cell lysates. IHC-P: Human brain tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2633Y -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68480の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro. -
配列類似性
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
ドメイン
The RGG-box domain is methylated. -
翻訳後修飾
Methylated. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 11100 Human
- Omim: 605800 Human
- SwissProt: Q9BUJ2 Human
- Unigene: 155218 Human
- Unigene: 718642 Human
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別名
- Adenovirus early region 1B associated protein 5 antibody
- Adenovirus early region 1B-associated protein 5 antibody
- E130317O14Rik antibody
see all
画像
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Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)All lanes : Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : HNRNPUL1 knockout HEK293T cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDaLanes 1-3: Merged signal (red and green). Green - ab68480 observed at 96 kDa. Red - loading control ab8245 observed at 36 kDa.
ab68480 Anti-E1B-AP5 antibody [EP2633Y] was shown to specifically react with E1B-AP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266142 (knockout cell lysate ab257993) was used. Wild-type and E1B-AP5 knockout samples were subjected to SDS-PAGE. ab68480 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.co.jp/ps/products/68/ab68480/Images/ab68480-82777-anti-e1b-ap5-antibody-ep2633y-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)ab68480 at 1/250 dilution staining E1B-AP5 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)](https://www.abcam.co.jp/ps/products/68/ab68480/Images/ab68480-82779-anti-e1b-ap5-antibody-ep2633y-western-blot.gif)
Western blot - Anti-E1B-AP5 antibody [EP2633Y] (ab68480)Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit, HRP conjugated at 1/2000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab68480 は 5 報の論文で使用されています。
- Batra J et al. Non-canonical proline-tyrosine interactions with multiple host proteins regulate Ebola virus infection. EMBO J 40:e105658 (2021). PubMed: 34260076
- Xiao R et al. Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell 178:107-121.e18 (2019). PubMed: 31251911
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708
- Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . PubMed: 25299183
- Massimelli MJ et al. Interplay between polyadenylate-binding protein 1 and Kaposi's sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA. J Virol 87:243-56 (2013). PubMed: 23077296