Anti-E tag 抗体 (ab3397)
Key features and details
- Rabbit polyclonal to E tag
- Suitable for: ICC/IF
- Reacts with: Species independent
- Isotype: IgG
製品の概要
-
製品名
Anti-E tag antibody
E tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to E tag -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to E tag conjugated to keyhole limpet haemocyanin.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3397の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR. -
別名
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-E tag antibody (ab3397)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)- fixed, permeabilized with 0.1%Triton X-100 in CHO cells transfected with 12-tags constructs (CHO-12 tags) labelling E tag with ab3397 at 1/1000 dilution, followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/100 dilution (Green) or Donkey Anti-rabbit IgG (H&L), Alexa Fluor594 antibody at 1/500 dilution (Red).
Nucleus was counterstained with DAPI (Blue).
Images also show parallel staining in parental CHO cell line.
-
Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreviewab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (12)
ab3397 は 12 報の論文で使用されています。
- Kaewchim K et al. Neutralizing and Enhancing Epitopes of the SARS-CoV-2 Receptor-Binding Domain (RBD) Identified by Nanobodies. Viruses 15:N/A (2023). PubMed: 37376552
- Glab-Ampai K et al. Human Superantibodies to 3CLpro Inhibit Replication of SARS-CoV-2 across Variants. Int J Mol Sci 23:N/A (2022). PubMed: 35743031
- Kaewchim K et al. Engineered Fully Human Single-Chain Monoclonal Antibodies to PIM2 Kinase. Molecules 26:N/A (2021). PubMed: 34770845
- McNutt PM et al. Neuronal delivery of antibodies has therapeutic effects in animal models of botulism. Sci Transl Med 13:N/A (2021). PubMed: 33408188
- Chekli Y et al. Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Sci Rep 10:15791 (2020). PubMed: 32978420